Mutations of the HFE Gene and the Risk of Hepatocellular Carcinoma

• Published in: Blood cells, molecules, & diseases molecules, & diseases, 1999-12, Vol.25 (6), p.350-353
• Main Authors: Racchi, Omar, Mangerini, Rosa, Rapezzi, Davide, Gaetani, Gian Franco, Nobile, Maria Teresa, Picciotto, Antonino, Ferraris, Anna Maria
• Format: Article
• Language: English
• Subjects: Aged; Carcinoma, Hepatocellular - etiology; Carcinoma, Hepatocellular - genetics; Female; Gene Frequency; Genes, MHC Class I; Hemochromatosis - blood; Hemochromatosis - complications; Hemochromatosis - genetics; Hemochromatosis Protein; hereditary hemochromatosis, HFE, C282Y, H63D, iron overload, hepatocellular carcinoma; Heterozygote; Histocompatibility Antigens Class I - blood; Histocompatibility Antigens Class I - genetics; HLA Antigens - blood; HLA Antigens - genetics; Humans; Iron Overload - blood; Iron Overload - complications; Iron Overload - genetics; Liver Cirrhosis - blood; Liver Cirrhosis - complications; Liver Cirrhosis - genetics; Liver Neoplasms - etiology; Liver Neoplasms - genetics; Male; Membrane Proteins; Middle Aged; Point Mutation; Risk Factors
• ISSN: 1079-9796; 1096-0961
• DOI: 10.1006/bcmd.1999.0263

The discovery of the C282Y and H63D point mutations in the hereditary hemochromatosis-associated HFE gene allows us to study the molecular basis of congenital and acquired iron overload disorders. In hereditary hemochromatosis an increased frequency of the C282Y and, to a lesser extent, of the H63D mutations has been established, but their role in other conditions associated with iron overload and their prevalence in the normal population are still under investigation. We sought to determine the presence of such mutations, and their possible involvement in the multi-step neoplastic transformation of the hepatocytes, in patients diagnosed with hepatocellular carcinoma, a frequent complication of iron-induced liver cirrhosis occurring in untreated hereditary hemochromatosis subjects. The frequency of the C282Y and H63D mutations was determined in DNA from 12 patients with hepatocellular carcinoma and with no clinical signs of hereditary hemochromatosis. The frequency of the mutations was also determined in 130 normal subjects. A germline C282Y mutation was found in none of the hepatocellular carcinoma patients; the frequency of the H63D mutation was not increased, compared to the 130 controls. The allele frequencies of the C282Y and H63D mutations in the normal population were 0.042 and 0.185, respectively. In conclusion, we suggest that the hereditary hemochromatosis-related mutations of the HFE gene do not play a significant role in the pathogenesis of hepatocellular carcinoma.