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Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth
The presence and expression of the multiple tbp genes encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negativ...
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Published in: | Archives of microbiology 2008-09, Vol.190 (3), p.309-318 |
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description | The presence and expression of the multiple tbp genes encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negative mutant strain R1. The only tbp gene located in the chromosome is tbpE, whereas all others are found in the plasmid DNA. Due to the dynamic nature of the plasmids in the Halobacterium strains, the copy numbers of the alternative tbp genes vary significantly. Five tbp genes (tbpA through tbpE) were present in the wild-type strain Hbt. salinarum PHH1. The tbpC gene of Hbt. salinarum PHH1 carried an ISH27-2 insertion element at the start of the reading frame that prevented the expression. All other tbp genes of PHH1 were expressed under aerobic and anaerobic growth conditions and quantitative RT-PCR yielded tbpE as dominant tbp transcript during the exponential growth phase. The plasmid deletion variant Hbt. salinarum PHH4 lacked all of the tbp genes except for tbpE and showed an altered growth behaviour compared to PHH1 wild-type in the stationary growth phase under anaerobic growth conditions. |
doi_str_mv | 10.1007/s00203-008-0383-5 |
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Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negative mutant strain R1. The only tbp gene located in the chromosome is tbpE, whereas all others are found in the plasmid DNA. Due to the dynamic nature of the plasmids in the Halobacterium strains, the copy numbers of the alternative tbp genes vary significantly. Five tbp genes (tbpA through tbpE) were present in the wild-type strain Hbt. salinarum PHH1. The tbpC gene of Hbt. salinarum PHH1 carried an ISH27-2 insertion element at the start of the reading frame that prevented the expression. All other tbp genes of PHH1 were expressed under aerobic and anaerobic growth conditions and quantitative RT-PCR yielded tbpE as dominant tbp transcript during the exponential growth phase. The plasmid deletion variant Hbt. salinarum PHH4 lacked all of the tbp genes except for tbpE and showed an altered growth behaviour compared to PHH1 wild-type in the stationary growth phase under anaerobic growth conditions.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-008-0383-5</identifier><identifier>PMID: 18506423</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Aerobiosis ; Alternative tbp genes ; Anaerobiosis ; Archaeal Proteins - genetics ; Base Sequence ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Chromosomes ; DNA Transposable Elements ; DNA, Archaeal - genetics ; Ecology ; Gene Expression Regulation, Archaeal ; Genes ; Genomes ; Growth conditions ; Halobacterium salinarum - genetics ; Halobacterium salinarum - growth & development ; ISH elements ; Life Sciences ; Microbial Ecology ; Microbiology ; Molecular Sequence Data ; Original Paper ; Plasmid variation ; Plasmids ; Plasmids - genetics ; Proteins ; Reverse Transcriptase Polymerase Chain Reaction ; RNA polymerase ; RNA, Archaeal - genetics ; TATA-Box Binding Protein - genetics ; TBP proteins ; Transcription factors ; Transcription Initiation Site</subject><ispartof>Archives of microbiology, 2008-09, Vol.190 (3), p.309-318</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-42e9f6a5d22a2160de70ea8a47211a376386631032ec0182f6f76b2a4f811cad3</citedby><cites>FETCH-LOGICAL-c393t-42e9f6a5d22a2160de70ea8a47211a376386631032ec0182f6f76b2a4f811cad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18506423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teufel, Katharina</creatorcontrib><creatorcontrib>Bleiholder, Anne</creatorcontrib><creatorcontrib>Griesbach, Tim</creatorcontrib><creatorcontrib>Pfeifer, Felicitas</creatorcontrib><title>Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>The presence and expression of the multiple tbp genes encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negative mutant strain R1. The only tbp gene located in the chromosome is tbpE, whereas all others are found in the plasmid DNA. Due to the dynamic nature of the plasmids in the Halobacterium strains, the copy numbers of the alternative tbp genes vary significantly. Five tbp genes (tbpA through tbpE) were present in the wild-type strain Hbt. salinarum PHH1. The tbpC gene of Hbt. salinarum PHH1 carried an ISH27-2 insertion element at the start of the reading frame that prevented the expression. All other tbp genes of PHH1 were expressed under aerobic and anaerobic growth conditions and quantitative RT-PCR yielded tbpE as dominant tbp transcript during the exponential growth phase. The plasmid deletion variant Hbt. salinarum PHH4 lacked all of the tbp genes except for tbpE and showed an altered growth behaviour compared to PHH1 wild-type in the stationary growth phase under anaerobic growth conditions.</description><subject>Aerobiosis</subject><subject>Alternative tbp genes</subject><subject>Anaerobiosis</subject><subject>Archaeal Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chromosomes</subject><subject>DNA Transposable Elements</subject><subject>DNA, Archaeal - genetics</subject><subject>Ecology</subject><subject>Gene Expression Regulation, Archaeal</subject><subject>Genes</subject><subject>Genomes</subject><subject>Growth conditions</subject><subject>Halobacterium salinarum - genetics</subject><subject>Halobacterium salinarum - growth & development</subject><subject>ISH elements</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Original Paper</subject><subject>Plasmid variation</subject><subject>Plasmids</subject><subject>Plasmids - genetics</subject><subject>Proteins</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA polymerase</subject><subject>RNA, Archaeal - genetics</subject><subject>TATA-Box Binding Protein - genetics</subject><subject>TBP proteins</subject><subject>Transcription factors</subject><subject>Transcription Initiation Site</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQQC1ERbeFD-ACFofeUsZ24jhHVAFFqsQBirhZs8l46ypxgu0I-vckZCUQh55syW-eLT_GXgq4FAD12wQgQRUApgBlVFE9YTtRKllALb8_ZTtQIAvTKHXKzlK6BxDSGPOMnQpTgS6l2rH8DaPH7MeQuA883xEf5j77qSee9xM_UKA_J513jiKFzK-xH_fYZop-HnjC3geM6y5H9IsGQ7d6fOT0a4qU0iLn3Rx9OPBDHH_mu-fsxGGf6MVxPWe3H95_vboubj5__HT17qZoVaNyUUpqnMaqkxKl0NBRDYQGy1oKgarWymitBChJLQgjnXa13kssnRGixU6ds4vNO8Xxx0wp28GnlvoeA41zsroppalNtYBv_gPvxzmG5W1WNI0qTWP0AokNauOYUiRnp-gHjA9WgF172K2HXXrYtYddxa-O4nk_UPd34hhgAeQGpGn9IIr_3PyI9fU25HC0eIg-2dsvEoQCaKSGqla_AZMXn9A</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Teufel, Katharina</creator><creator>Bleiholder, Anne</creator><creator>Griesbach, Tim</creator><creator>Pfeifer, Felicitas</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth</title><author>Teufel, Katharina ; Bleiholder, Anne ; Griesbach, Tim ; Pfeifer, Felicitas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-42e9f6a5d22a2160de70ea8a47211a376386631032ec0182f6f76b2a4f811cad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Aerobiosis</topic><topic>Alternative tbp genes</topic><topic>Anaerobiosis</topic><topic>Archaeal Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Chromosomes</topic><topic>DNA Transposable Elements</topic><topic>DNA, Archaeal - genetics</topic><topic>Ecology</topic><topic>Gene Expression Regulation, Archaeal</topic><topic>Genes</topic><topic>Genomes</topic><topic>Growth conditions</topic><topic>Halobacterium salinarum - genetics</topic><topic>Halobacterium salinarum - growth & development</topic><topic>ISH elements</topic><topic>Life Sciences</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Original Paper</topic><topic>Plasmid variation</topic><topic>Plasmids</topic><topic>Plasmids - genetics</topic><topic>Proteins</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA polymerase</topic><topic>RNA, Archaeal - genetics</topic><topic>TATA-Box Binding Protein - genetics</topic><topic>TBP proteins</topic><topic>Transcription factors</topic><topic>Transcription Initiation Site</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teufel, Katharina</creatorcontrib><creatorcontrib>Bleiholder, Anne</creatorcontrib><creatorcontrib>Griesbach, Tim</creatorcontrib><creatorcontrib>Pfeifer, Felicitas</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teufel, Katharina</au><au>Bleiholder, Anne</au><au>Griesbach, Tim</au><au>Pfeifer, Felicitas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>190</volume><issue>3</issue><spage>309</spage><epage>318</epage><pages>309-318</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>The presence and expression of the multiple tbp genes encoding TATA-box binding proteins (TBPs) was investigated in various strains and mutants of the archaeon Halobacterium salinarum. Six genes, tbpA through tbpF, are present in the genome of Hbt. salinarum NRC-1 and also in the gas vesicle negative mutant strain R1. The only tbp gene located in the chromosome is tbpE, whereas all others are found in the plasmid DNA. Due to the dynamic nature of the plasmids in the Halobacterium strains, the copy numbers of the alternative tbp genes vary significantly. Five tbp genes (tbpA through tbpE) were present in the wild-type strain Hbt. salinarum PHH1. The tbpC gene of Hbt. salinarum PHH1 carried an ISH27-2 insertion element at the start of the reading frame that prevented the expression. All other tbp genes of PHH1 were expressed under aerobic and anaerobic growth conditions and quantitative RT-PCR yielded tbpE as dominant tbp transcript during the exponential growth phase. The plasmid deletion variant Hbt. salinarum PHH4 lacked all of the tbp genes except for tbpE and showed an altered growth behaviour compared to PHH1 wild-type in the stationary growth phase under anaerobic growth conditions.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18506423</pmid><doi>10.1007/s00203-008-0383-5</doi><tpages>10</tpages></addata></record> |
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subjects | Aerobiosis Alternative tbp genes Anaerobiosis Archaeal Proteins - genetics Base Sequence Biochemistry Biomedical and Life Sciences Biotechnology Cell Biology Chromosomes DNA Transposable Elements DNA, Archaeal - genetics Ecology Gene Expression Regulation, Archaeal Genes Genomes Growth conditions Halobacterium salinarum - genetics Halobacterium salinarum - growth & development ISH elements Life Sciences Microbial Ecology Microbiology Molecular Sequence Data Original Paper Plasmid variation Plasmids Plasmids - genetics Proteins Reverse Transcriptase Polymerase Chain Reaction RNA polymerase RNA, Archaeal - genetics TATA-Box Binding Protein - genetics TBP proteins Transcription factors Transcription Initiation Site |
title | Variations in the multiple tbp genes in different Halobacterium salinarum strains and their expression during growth |
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