l-Carnitine protects against apoptosis of murine MC3T3-E1 osteoblastic cells

l-Carnitine (LC), an amino acid with a major role in cellular energy metabolism, has positive effects on bone metabolism. However, the effect of LC on apoptosis of osteoblast in vitro has not been reported. The aim of this study was to investigate the action of LC on apoptosis of mouse osteoblastic...

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Bibliographic Details
Published in:Amino acids 2008-08, Vol.35 (2), p.419-423
Main Authors: Xie, H, Tang, S.-Y, Li, H, Luo, X.-H, Yuan, L.-Q, Wang, D, Liao, E.-Y
Format: Article
Language:English
Subjects:
Analytical Chemistry
Animals
Apoptosis
Apoptosis - drug effects
bcl-2-Associated X Protein - analysis
Biochemical Engineering
Biochemistry
Biomedical and Life Sciences
Blotting, Western
Carnitine - pharmacology
Caspases - drug effects
Caspases - metabolism
Cell Line
Cells
Culture Media - pharmacology
Cytochromes c - analysis
Dexamethasone - antagonists & inhibitors
Dexamethasone - pharmacology
Dose-Response Relationship, Drug
Enzymes
Glucocorticoid
l-Carnitine
Life Sciences
Mice
Neurobiology
Osteoblast
Osteoblasts - cytology
Osteoblasts - drug effects
Proteins
Proteomics
Proto-Oncogene Proteins c-bcl-2 - analysis
Structure-Activity Relationship
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Summary:l-Carnitine (LC), an amino acid with a major role in cellular energy metabolism, has positive effects on bone metabolism. However, the effect of LC on apoptosis of osteoblast in vitro has not been reported. The aim of this study was to investigate the action of LC on apoptosis of mouse osteoblastic cell line MC3T3-E1. Cell apoptosis was measured by sandwich-enzyme-immunoassay. Release of cytochrome c from mitochondria into cytosol and Bcl-2, Bax protein levels were determined by Western blot analysis. The enzyme substrate was used to assess the activation of caspase-3 and caspase-9. LC inhibited MC3T3-E1 cell apoptosis induced by serum deprivation. Our study also shows that LC decreased cytochrome c release and caspase-3 and caspase-9 activation in serum-deprived MC3T3-E1 cells. Furthermore, LC protected against MC3T3-E1 cell apoptosis induced by the glucocorticoid (GC) dexamethasone (Dex).
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-007-0598-9