Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging

Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope...

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Bibliographic Details
Published in:Optics letters 2008-08, Vol.33 (16), p.1887-1889
Main Authors: ROBINSON, Tom, VALLURI, Prashant, DE MELLO, Andrew J, FRENCH, Paul M. W, MANNING, Hugh B, OWEN, Dylan M, MUNRO, Ian, TALBOT, Clifford B, DUNSBY, Christopher, ECCLESTON, John F, BALDWIN, Geoff S, NEIL, Mark A. A
Format: Article
Language:English
Subjects:
Applied fluid mechanics
Biological and medical sciences
Calibration
Equipment Design
Exact sciences and technology
Fluid dynamics
Fluidics
Fluorescence
Fundamental and applied biological sciences. Psychology
Fundamental areas of phenomenology (including applications)
General aspects, investigation technics, apparatus
Imaging, Three-Dimensional
Kinetics
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Physics
Time Factors
Tissues, organs and organisms biophysics
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Summary:Fluorescence lifetime imaging (FLIM) is used to quantitatively map the concentration of a small molecule in three dimensions in a microfluidic mixing device. The resulting experimental data are compared with computational fluid-dynamics (CFD) simulations. A line-scanning semiconfocal FLIM microscope allows the full mixing profile to be imaged in a single scan with submicrometer resolution over an arbitrary channel length from the point of confluence. Following experimental and CFD optimization, mixing times down to 1.3+/-0.4 ms were achieved with the single-layer microfluidic device.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.33.001887