Characterization of cytostatically active glycosphingolipids isolated from thioglycollate-elicited murine macrophages

Two acidic glycosphingolipids (gangliosides) derived from mouse macrophage membranes and separated by thin-layer chromatography have a strong cytostatic effect on human and mouse tumor cells. The structure of the two gangliosides, named M phi G1 and M phi G2, was elucidated by application of physico...

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Bibliographic Details
Published in:IUBMB life 1999-09, Vol.48 (3), p.353-358
Main Authors: Schaade, L, Ritter, K, Schiebel, H M, Thomssen, R, Kleines, M
Format: Article
Language:English
Subjects:
Animals
Glycosphingolipids - analysis
Glycosphingolipids - metabolism
Humans
Macrophage Activation
Macrophages, Peritoneal - metabolism
Mice
Thioglycolates
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Summary:Two acidic glycosphingolipids (gangliosides) derived from mouse macrophage membranes and separated by thin-layer chromatography have a strong cytostatic effect on human and mouse tumor cells. The structure of the two gangliosides, named M phi G1 and M phi G2, was elucidated by application of physicochemical and immunochemical methods. Gas chromatography and mass spectrometry of M phi G1 and M phi G2 classified them as isomeric monosialogangliosides with ceramide moieties composed of sphingosine as the long-chain base, C16 and C18 fatty acids, respectively, and a lacto-tetraose backbone. For M phi G1, additional immunochemical findings led to the proposed structure IV3NeuAc-nLcOse4Cer. The immunochemical reactions of M phi G2 suggest a branched structure for the oligosaccharide moiety.
ISSN:1521-6543
1521-6551
DOI:10.1080/152165499307099