Biochemical and structural characterisation of membrane-containing icosahedral dsDNA bacteriophages infecting thermophilic Thermus thermophilus

Abstract Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of t...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2008-09, Vol.379 (1), p.10-19
Main Authors: Jaatinen, S.T, Happonen, L.J, Laurinmäki, P, Butcher, S.J, Bamford, D.H
Format: Article
Language:English
Subjects:
Bacteria
Bacteriophage
Bacteriophages - chemistry
Bacteriophages - classification
Bacteriophages - isolation & purification
Bacteriophages - ultrastructure
Cryoelectron Microscopy
Freshwater
Hot Springs - virology
Infectious Disease
Lipids - analysis
Membrane-containing
Microscopy, Electron, Transmission
Models, Molecular
P23-65H
P23-72
P23-77
Protein Structure, Quaternary
Protein Structure, Tertiary
Tectiviridae
Tectiviridae - chemistry
Tectiviridae - classification
Tectiviridae - isolation & purification
Tectiviridae - ultrastructure
Thermophilic
Thermus
Thermus thermophilus
Thermus thermophilus - virology
Viral Plaque Assay
Viral Structural Proteins - isolation & purification
Virion - chemistry
Virion - ultrastructure
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Summary:Abstract Icosahedral dsDNA viruses isolated from hot springs and proposed to belong to the Tectiviridae family infect the Gram-negative thermophilic Thermus thermophilus bacterium. Seven such viruses were obtained from the Promega Corporation collection. The structural protein patterns of three of these viruses, growing to a high titer, appeared very similar but not identical. The most stable virus, P23-77, was chosen for more detailed studies. Analysis of highly purified P23-77 by thin layer chromatography for neutral lipids showed lipid association with the virion. Cryo-EM based three-dimensional image reconstruction of P23-77 to 1.4 nm resolution revealed an icosahedrally-ordered protein coat, with spikes on the vertices, and an internal membrane. The capsid architecture of P23-77 is most similar to that of the archaeal virus SH1. These findings further complicate the grouping of icosahedrally-symmetric viruses containing an inner membrane. We propose a single superfamily or order with members in several viral families.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2008.06.023