Sedimentation field-flow fractionation application to Toxoplasma gondii separation and purification

Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii, an intracellular protozoa of micronic size range (4–10 μm). Its classical purification processes are complex and often associated with low recovery. All investigation procedures concerning this parasite require its isolation and purif...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1999-07, Vol.20 (3), p.503-512
Main Authors: Bouamrane, Fawzi, Assidjo, N.Emmanuel, Bouteille, Bernard, Dreyfuss, Marie F, Dardé, Marie L, Cardot, Philippe J.P
Format: Article
Language:English
Subjects:
Animals
Ascitic Fluid - cytology
Ascitic Fluid - parasitology
Biological and medical sciences
Cell Count
Cell Separation - instrumentation
Cell Separation - methods
Chemical Fractionation - instrumentation
Chemical Fractionation - methods
Chromatography - instrumentation
Chromatography - methods
Erythrocytes
Erythrocytes - cytology
Erythrocytes - parasitology
Evaluation Studies as Topic
Female
Field-flow fractionation
Fundamental and applied biological sciences. Psychology
General aspects and techniques
Gravitation
Humans
Mice
Parasitology - instrumentation
Parasitology - methods
Particle Size
Protozoa
Reproducibility of Results
Sensitivity and Specificity
Size fractionation
Space life sciences
Toxoplasma - growth & development
Toxoplasma - isolation & purification
Toxoplasma gondii
Toxoplasmosis - parasitology
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Summary:Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii, an intracellular protozoa of micronic size range (4–10 μm). Its classical purification processes are complex and often associated with low recovery. All investigation procedures concerning this parasite require its isolation and purification from at least the mouse ascitic fluid. For this purpose, a recently developed laboratory technology was used, i.e. sedimentation field-flow fractionation. This chromatographic-like separation technology was demonstrated to be particularly selective for isolation and separation of micron-sized biological particles. Sedimentation field-flow fractionation operated on the steric-hyperlayer mode was used to isolate the parasite from the remanent ascitic contaminants of different origins and from red blood cells. With this technology, 86% recovery with 97% viability was obtained in less than 30 min.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(99)00049-7