Monitoring in vitro experiments using microdialysis sampling on-line with mass spectrometry

A method has been developed for the real-time analysis of components in in vitro reactions by the on-line combination of microdialysis sampling (MD) with tandem mass spectrometry (MS/MS) and single stage mass spectrometry (MS). Apparatus and parameters associated with the integration have been studi...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1999-06, Vol.20 (1), p.115-128
Main Authors: Kerns, Edward H, Volk, Kevin J, Klohr, Steven E, Lee, Mike S
Format: Article
Language:English
Subjects:
Acetates
Acetic Acid
Analysis
Animals
Biological and medical sciences
Calibration
Enzyme reaction monitoring
General pharmacology
Mass Spectrometry - standards
Medical sciences
Metabolite
Microdialysis
Microdialysis - standards
Online Systems
Pharmacology. Drug treatments
Protein binding
Pyrimidines - analysis
Pyrimidines - blood
Quality Control
Rats
Reproducibility of Results
Serotonin Receptor Agonists - analysis
Serotonin Receptor Agonists - blood
Substance P - analysis
Substance P - blood
Tandem mass spectrometry
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Summary:A method has been developed for the real-time analysis of components in in vitro reactions by the on-line combination of microdialysis sampling (MD) with tandem mass spectrometry (MS/MS) and single stage mass spectrometry (MS). Apparatus and parameters associated with the integration have been studied. Analytical figures of merit for the drug gepirone have been determined. The qualitative ‘limit of identification’ was found to be 100 ng/ml and 200 ng/ml for methods using thermospray and electrospray MS interfaces, respectively. Using this approach, monitoring of in vitro experiments involving drug metabolites, enzymatic reactions, and ligand-protein binding interactions were performed.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(99)00003-5