Characterization of the glycine betaine biosynthetic genes in the moderately halophilic bacterium Halobacillus dabanensis D-8(T)

The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8(T). Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb...

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Bibliographic Details
Published in:Current microbiology 2008-10, Vol.57 (4), p.306-311
Main Authors: Gu, Zhi Jing, Wang, Lei, Le Rudulier, Daniel, Zhang, Bo, Yang, Su Sheng
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillaceae - genetics
Bacillaceae - growth & development
Bacillaceae - metabolism
Bacillaceae - physiology
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Betaine - metabolism
Choline - metabolism
Culture Media
DNA, Bacterial - analysis
Escherichia coli - genetics
Gene Expression Regulation, Bacterial
Magnetic Resonance Spectroscopy - methods
Molecular Sequence Data
Multigene Family
Polymerase Chain Reaction
Sequence Analysis, DNA
Sodium Chloride - metabolism
Sodium Chloride - pharmacology
Water-Electrolyte Balance
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Summary:The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8(T). Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb fragment. After heterologous expression of gbsAB in the Escherichia coli mutant strain PD141, the transformed cells were able to grow in a selective M63 medium containing 0.7 M NaCl and 1 mM choline, in contrast to the mutant strain. Glycine betaine biosynthesis was restored and its accumulation was confirmed by using (13)C nuclear magnetic resonance spectroscopy.
ISSN:0343-8651
DOI:10.1007/s00284-008-9194-9