Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis

Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related st...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2008-09, Vol.35 (9), p.931-945
Main Authors: Ritacco, Frank V, Eveleigh, Douglas E
Format: Article
Language:English
Subjects:
Acetogenins - chemistry
Acetogenins - metabolism
Bacterial Proteins - genetics
bacterial spores
Base Sequence
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbohydrate Metabolism
Carbohydrates
Chromatography
Chromatography, High Pressure Liquid
Cluster Analysis
Communication
Culture Media
DNA fingerprinting
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Inorganic Chemistry
Kinases
Life Sciences
Liquid chromatography
Metabolites
metabolomics
Microbiology
Molecular Sequence Data
molecular systematics
Natural products
nucleotide sequences
Original Paper
phaeochromycin
Phenotype
phenotypic variation
Phylogenetics
pigments
polyketides
ribosomal RNA
Ribotyping
RNA, Ribosomal, 16S - genetics
Sequence Alignment
sequence analysis
Software
soil bacteria
Species Specificity
Streptomyces
Streptomyces - classification
Streptomyces - genetics
Streptomyces - metabolism
Streptomyces - physiology
Streptomyces ederensis
Streptomyces phaeochromogenes
Studies
Taxonomy
Technical services
Tumor necrosis factor-TNF
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Description
Summary:Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related strains from major culture collections by 16S rRNA gene sequence, ribotype, HPLC-MS metabolite profile, and rpoB sequence. Using BioNumerics software, genetic and chemical fingerprint data were integrated via multivariate cluster analysis into a single, robust comparison. Based upon this analysis, strain LL-P018 is very closely related to the type strains of both S. phaeochromogenes and Streptomyces ederensis, indicating that these two types may in fact represent a single species. This novel comparative multi-cluster analysis is most useful for clarifying relationships between closely related species.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-008-0367-0