A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase

Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-08, Vol.56 (16), p.6835-6843
Main Authors: Cheng, Hsueh-Ling, Huang, Hsin-Kai, Chang, Chi-I, Tsai, Chung-Pao, Chou, Chang-Hung
Format: Article
Language:English
Subjects:
AMP-activated protein kinase
AMP-Activated Protein Kinases
Analytical Methods
Animals
Biological and medical sciences
Cell Line
chemical constituents of plants
Enzyme Activation - drug effects
Fish and seafood industries
FL83B cells
Food industries
Fundamental and applied biological sciences. Psychology
Glucose - metabolism
glycemic effect
Hypoglycemic Agents - pharmacology
Insulin Resistance
Liver
Mice
Momordica charantia
Momordica charantia - chemistry
Multienzyme Complexes - metabolism
Plant Extracts - chemistry
Plant Extracts - pharmacology
Plant Stems - chemistry
protein kinases
Protein-Serine-Threonine Kinases - metabolism
screening
stems
triterpenoids
Tumor Necrosis Factor-alpha - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf800801k