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A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase
Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients...
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| Published in: | Journal of agricultural and food chemistry 2008-08, Vol.56 (16), p.6835-6843 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a405t-1664348dd1dfe40a223c99a757f00ac14058d5e285cbfa6fbea9921e71fd281b3 |
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| cites | cdi_FETCH-LOGICAL-a405t-1664348dd1dfe40a223c99a757f00ac14058d5e285cbfa6fbea9921e71fd281b3 |
| container_end_page | 6843 |
| container_issue | 16 |
| container_start_page | 6835 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 56 |
| creator | Cheng, Hsueh-Ling Huang, Hsin-Kai Chang, Chi-I Tsai, Chung-Pao Chou, Chang-Hung |
| description | Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase. |
| doi_str_mv | 10.1021/jf800801k |
| format | article |
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The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf800801k</identifier><identifier>PMID: 18656931</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>AMP-activated protein kinase ; AMP-Activated Protein Kinases ; Analytical Methods ; Animals ; Biological and medical sciences ; Cell Line ; chemical constituents of plants ; Enzyme Activation - drug effects ; Fish and seafood industries ; FL83B cells ; Food industries ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; glycemic effect ; Hypoglycemic Agents - pharmacology ; Insulin Resistance ; Liver ; Mice ; Momordica charantia ; Momordica charantia - chemistry ; Multienzyme Complexes - metabolism ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Plant Stems - chemistry ; protein kinases ; Protein-Serine-Threonine Kinases - metabolism ; screening ; stems ; triterpenoids ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Journal of agricultural and food chemistry, 2008-08, Vol.56 (16), p.6835-6843</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a405t-1664348dd1dfe40a223c99a757f00ac14058d5e285cbfa6fbea9921e71fd281b3</citedby><cites>FETCH-LOGICAL-a405t-1664348dd1dfe40a223c99a757f00ac14058d5e285cbfa6fbea9921e71fd281b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20611239$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18656931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Hsueh-Ling</creatorcontrib><creatorcontrib>Huang, Hsin-Kai</creatorcontrib><creatorcontrib>Chang, Chi-I</creatorcontrib><creatorcontrib>Tsai, Chung-Pao</creatorcontrib><creatorcontrib>Chou, Chang-Hung</creatorcontrib><title>A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.</description><subject>AMP-activated protein kinase</subject><subject>AMP-Activated Protein Kinases</subject><subject>Analytical Methods</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>chemical constituents of plants</subject><subject>Enzyme Activation - drug effects</subject><subject>Fish and seafood industries</subject><subject>FL83B cells</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>glycemic effect</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Insulin Resistance</subject><subject>Liver</subject><subject>Mice</subject><subject>Momordica charantia</subject><subject>Momordica charantia - chemistry</subject><subject>Multienzyme Complexes - metabolism</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Stems - chemistry</subject><subject>protein kinases</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>screening</subject><subject>stems</subject><subject>triterpenoids</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpt0cFuEzEQBuAVAtG0cOAFwBeQOCzY6_Wu95iGUiJaNSLthYs1scet06wd7N0KnoWXxSghvXCyrPk8tv8pileMfmC0Yh_XVlIqKbt_UkyYqGgpGJNPiwnNxVKKhh0VxymtaUaipc-LIyYb0XScTYrfUzLDzaY8hYSGLHVE9M7fkrlBPzjrMJFZ6Ldh9CYRG0NPhjskywF7Eiy5DH2Ixmkg-g4i5BOQ6zCQqweMOvRI5j6NG-fJN0wuDeA1EvCGTPXgHmBAMr1clP82hixiGDDrr87n97wonlnYJHy5X0-Km89n17Mv5cXV-Xw2vSihpmIoWdPUvJbGMGOxplBVXHcdtKK1lIJmGUkjsJJCryw0doXQdRXDlllTSbbiJ8W7Xd9tDD9GTIPqXdI5FfAYxqSarq5lw9sM3--gjiGliFZto-sh_lKMqr-TUIdJZPt633Rc9Wge5T76DN7uASQNG5vj0y4dXEUbxireZVfuXM4Pfx7qEO9V0_JWqOvFUvHzT0J-X3DFs3-z8xaCgtuYe94sK8o4ZYI3oqsfbwad1DqM0ed0__OFP9Potec</recordid><startdate>20080827</startdate><enddate>20080827</enddate><creator>Cheng, Hsueh-Ling</creator><creator>Huang, Hsin-Kai</creator><creator>Chang, Chi-I</creator><creator>Tsai, Chung-Pao</creator><creator>Chou, Chang-Hung</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080827</creationdate><title>A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase</title><author>Cheng, Hsueh-Ling ; Huang, Hsin-Kai ; Chang, Chi-I ; Tsai, Chung-Pao ; Chou, Chang-Hung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a405t-1664348dd1dfe40a223c99a757f00ac14058d5e285cbfa6fbea9921e71fd281b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>AMP-activated protein kinase</topic><topic>AMP-Activated Protein Kinases</topic><topic>Analytical Methods</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>chemical constituents of plants</topic><topic>Enzyme Activation - drug effects</topic><topic>Fish and seafood industries</topic><topic>FL83B cells</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>glycemic effect</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Insulin Resistance</topic><topic>Liver</topic><topic>Mice</topic><topic>Momordica charantia</topic><topic>Momordica charantia - chemistry</topic><topic>Multienzyme Complexes - metabolism</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Stems - chemistry</topic><topic>protein kinases</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>screening</topic><topic>stems</topic><topic>triterpenoids</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheng, Hsueh-Ling</creatorcontrib><creatorcontrib>Huang, Hsin-Kai</creatorcontrib><creatorcontrib>Chang, Chi-I</creatorcontrib><creatorcontrib>Tsai, Chung-Pao</creatorcontrib><creatorcontrib>Chou, Chang-Hung</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheng, Hsueh-Ling</au><au>Huang, Hsin-Kai</au><au>Chang, Chi-I</au><au>Tsai, Chung-Pao</au><au>Chou, Chang-Hung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2008-08-27</date><risdate>2008</risdate><volume>56</volume><issue>16</issue><spage>6835</spage><epage>6843</epage><pages>6835-6843</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Treatment of insulin resistance is a critical strategy in the prevention and management of type 2 diabetes. The crude extracts from all parts of Momordica charantia L. have been reported by many studies for the effective treatment of diabetes and related complications. However, the exact ingredients responsible for the hypoglycemic effect and the underlying mechanism of their actions have not been well characterized because of the lack of a proper assay and screening system. A new cell-based, nonradioactive, and nonfluorescent screening method was demonstrated in this study to screen for natural products from the stem of M. charantia, aiming to identify hypoglycemic components that can overcome cellular insulin resistance. The results suggest triterpenoids being potential hypoglycemic components of the plant and the mechanism underlying their action involving AMP-activated protein kinase.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>18656931</pmid><doi>10.1021/jf800801k</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2008-08, Vol.56 (16), p.6835-6843 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | AMP-activated protein kinase AMP-Activated Protein Kinases Analytical Methods Animals Biological and medical sciences Cell Line chemical constituents of plants Enzyme Activation - drug effects Fish and seafood industries FL83B cells Food industries Fundamental and applied biological sciences. Psychology Glucose - metabolism glycemic effect Hypoglycemic Agents - pharmacology Insulin Resistance Liver Mice Momordica charantia Momordica charantia - chemistry Multienzyme Complexes - metabolism Plant Extracts - chemistry Plant Extracts - pharmacology Plant Stems - chemistry protein kinases Protein-Serine-Threonine Kinases - metabolism screening stems triterpenoids Tumor Necrosis Factor-alpha - pharmacology |
| title | A Cell-Based Screening Identifies Compounds from the Stem of Momordica charantia that Overcome Insulin Resistance and Activate AMP-Activated Protein Kinase |
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