Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2008-06, Vol.80 (1), p.69-73
Main Authors: NUNAN, Linda M, PANTOJA, Carlos, LIGHTNER, Donald V
Format: Article
Language:English
Subjects:
Alphaproteobacteria - genetics
Alphaproteobacteria - isolation & purification
Animal aquaculture
Animal productions
Animals
Biological and medical sciences
Crustacea
Decapoda
Fundamental and applied biological sciences. Psychology
Hepatopancreas - microbiology
Invertebrate aquaculture
Invertebrates
Marine
Pathology
Penaeidae - microbiology
Polymerase Chain Reaction
Sensitivity and Specificity
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Summary:Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao01908