Establishment and Analysis of Biological Characteristics of a Human Duodenal Carcinoma Cell Line, WDC-1

Background: Duodenal carcinoma is very rare and its culture cell lines have rarely been established. Methods: Tumor cells separated from a surgically resected primary tumor of duodenal carcinoma were put into culture. The patient was an 81-year-old female and had metastatic lymph nodes. We investiga...

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Bibliographic Details
Published in:Japanese journal of clinical oncology 1999-12, Vol.29 (12), p.596-599
Main Authors: Tani, Masaji, Tanimura, Hiroshi, Terasawa, Hiroshi, Yamade, Naohisa, Shono, Yoshiharu, Yamaue, Hiroki
Format: Article
Language:English
Subjects:
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Adenocarcinoma - secondary
adhesion molecule
Aged
Aged, 80 and over
Animals
CA-19-9 Antigen - metabolism
carcinoembryonic antigen
Carcinoembryonic Antigen - biosynthesis
duodenal carcinoma
Duodenal Neoplasms - metabolism
Duodenal Neoplasms - pathology
Female
Humans
Integrins - metabolism
Lymphatic Metastasis
Mice
Mice, Inbred BALB C
Mice, Nude
neoadjuvant chemotherapy
Neoplasm Transplantation
tumor cell line
Tumor Cells, Cultured
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:Background: Duodenal carcinoma is very rare and its culture cell lines have rarely been established. Methods: Tumor cells separated from a surgically resected primary tumor of duodenal carcinoma were put into culture. The patient was an 81-year-old female and had metastatic lymph nodes. We investigated the biological characteristics of the culture cells including in vitro cell kinetics, karyotype, expression of tumor markers and integrins and tumorigenicity and histology in nude mice. Results: A new cell line, designated WDC-1, was established. This duodenal carcinoma cell line proliferated in a monolayered sheet with a doubling time of 50 h. The histological findings of the xenograft in nude mice were similar to those of the primary tumor. WDC-1 cells produced carcinoembryonic antigen and expressed 1 integrin and very late antigen (VLA)-4d in vitro. Conclusions: A duodenal carcinoma cell line was established, which is rare and may contribute to progress in understanding the biological features of duodenal cancer.
ISSN:0368-2811
1465-3621
DOI:10.1093/jjco/29.12.596