Histopathological and ultrastructural features of Koi herpesvirus (KHV)-infected carp Cyprinus carpio, and the morphology and morphogenesis of KHV

Epizootics of Koi herpesvirus (KHV) cause mass mortalities in koi carp and common carp worldwide. We used a newly developed 'per-gill infection' procedure with live KHV, and then conducted detailed histopathological and ultrastructural studies of KHV-infected cells including an examination...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2008-06, Vol.80 (1), p.1-11
Main Authors: MIYAZAKIL, Teruo, KUZUYA, Yoshitaka, YASUMOTO, Shinya, YASUDA, Masahiro, KOBAYASHI, Tatsuya
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
Biological and medical sciences
Carps - virology
Cyprinus carpio
Fish Diseases - pathology
Fish Diseases - virology
Freshwater
Herpesviridae
Herpesviridae - growth & development
Herpesviridae - physiology
Herpesviridae - ultrastructure
Herpesviridae Infections - pathology
Herpesviridae Infections - veterinary
Infectious diseases
koi herpesvirus
Medical sciences
Microscopy, Electron, Transmission
Viral diseases
Virus Assembly
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Summary:Epizootics of Koi herpesvirus (KHV) cause mass mortalities in koi carp and common carp worldwide. We used a newly developed 'per-gill infection' procedure with live KHV, and then conducted detailed histopathological and ultrastructural studies of KHV-infected cells including an examination of the morphology and morphogenesis of KHV. The primary target of KHV was respiratory epithelial cells of the gill lamellae, and release of virions from infected epithelial cells resulted in a systemic infection affecting the kidney, spleen, heart, brain and liver. The pathognomonic feature of infected cells was the formation of intranuclear inclusion bodies with marginal hyperchromatosis in the nucleus. Within the nucleus, assembly of capsids and nucleocapsids and an increase in filamentous nucleoproteins were evident. Enveloped nucleocapsids budded from the inner nuclear membrane into the perinuclear space. De-enveloped nucleocapsids were translocated in the cytoplasm to be embedded within inclusion bodies where tegumentation of the nucleocapsid occurred. Enveloped virions that had budded into intracytoplasmic vesicles and virions located extracellularly were composed of an electron-dense core, surrounded in turn by the capsid, the tegument and finally an envelope with projections. The morphology and morphogenesis of KHV were the same as those of viruses within the family Herpesviridae.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao01929