Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis

Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-γ- d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respe...

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Bibliographic Details
Published in:FEMS microbiology letters 2008-08, Vol.285 (2), p.220-226
Main Authors: Cachat, Elise, Barker, Margaret, Read, Timothy D, Priest, Fergus G
Format: Article
Language:English
Subjects:
Antibodies
Antibodies, Bacterial - immunology
Bacillus anthracis
Bacillus anthracis - immunology
Bacillus cereus
Bacillus cereus - genetics
Bacillus thuringiensis
Bacillus thuringiensis - genetics
Bacillus thuringiensis - immunology
Bacillus thuringiensis - metabolism
Bacterial Capsules - biosynthesis
Bacterial Capsules - immunology
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Biosynthesis
Butterflies & moths
capsule
Cereus
Fluorescence
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Genotype
Glutamic acid
Microbiology
MLST
Morphology, structure, chemical composition
Multigene Family
Multilocus sequence typing
Phylogeny
Plasmids
polyglutamic acid
Polyglutamic Acid - biosynthesis
Polyglutamic Acid - immunology
Sequence Analysis, DNA
Sequence Homology
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Summary:Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-γ- d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the B. anthracis capBCADE cluster were present on a plasmid (pAJ1-1). Strain BGSC 4AJ1, together with five strains of Bacillus cereus that hybridized to a PGA cap gene probe, were analyzed phylogenetically using six housekeeping genes of a B. cereus multilocus sequence typing scheme. Bacillus thuringiensis BGSC 4AJ1 shared four identical alleles with B. anthracis and was the second most closely related to this bacterium of the 674 isolates in the multilocus sequence typing database. The other cap⁺ strains were distributed among various lineages of Clade 1 of the B. cereus group.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2008.01231.x