Structural characterization of neutral and anionic glucans from Mesorhizobium loti

The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic β-(1→2)- d-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fract...

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Bibliographic Details
Published in:Carbohydrate research 2008-09, Vol.343 (14), p.2422-2427
Main Authors: Kawaharada, Yasuyuki, Kiyota, Hiromasa, Eda, Shima, Minamisawa, Kiwamu, Mitsui, Hisayuki
Format: Article
Language:English
Subjects:
Alphaproteobacteria - chemistry
Carbohydrate Conformation
Carbohydrate Sequence
Cyclic glucan
Glucans - chemistry
Glucans - isolation & purification
Glycerophosphates - chemistry
Glycerophosphoryl substituent
Magnetic Resonance Spectroscopy
Mesorhizobium loti
Molecular Sequence Data
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Succinic Acid - chemistry
Succinyl substituent
β-(1→2)-Glycosidic linkage
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Summary:The periplasmic glucans of Mesorhizobium loti were isolated and separated into fractions according to their acidity. NMR spectroscopy confirmed their backbone structure to be a cyclic β-(1→2)- d-glucan as in the case of other rhizobia, and revealed no non-glycosidic substituents in the neutral fraction, and glycerophosphoryl and succinyl residues as major and minor substituents, respectively, in the anionic fractions. MALDI-TOF mass spectrometry showed that the anionic glucans contain one, two, or three such substituents per molecule according to their acidity, and, in contrast, that all the anionic subfractions have a similar size distribution to that of the neutral glucans, where molecules composed of 20–24 glucosyl residues are predominant. These results clarify the periplasmic glucan composition in terms of charge-to-mass ratios in M. loti cells.
ISSN:0008-6215
1873-426X
DOI:10.1016/j.carres.2008.07.007