The antimicrobial peptides piscidins are stored in the granules of professional phagocytic granulocytes of fish and are delivered to the bacteria-containing phagosome upon phagocytosis

Antimicrobial peptides (AMPs) are increasingly recognized as a critical first line of defence against many pathogens. The genes encoding these peptides are expressed in numerous tissue and cell types from a wide variety of different species including mammals, amphibians, fish, and insects. In this s...

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Bibliographic Details
Published in:Developmental and comparative immunology 2008, Vol.32 (12), p.1531-1538
Main Authors: Mulero, Iván, Noga, Edward J., Meseguer, José, García-Ayala, Alfonsa, Mulero, Victoriano
Format: Article
Language:English
Subjects:
Acidophilic granulocytes
Animals
Antimicrobial Cationic Peptides - metabolism
Bacteria
Bacteria - immunology
Cytoplasmic Granules - metabolism
Cytoplasmic Granules - microbiology
Evolution
Fish Proteins - immunology
Fish Proteins - metabolism
Granulocytes - cytology
Granulocytes - metabolism
Granulocytes - microbiology
Immunohistochemistry
Mast cells
Mast Cells - cytology
Mast Cells - metabolism
Mast Cells - microbiology
Perciformes
Phagocytes - cytology
Phagocytes - metabolism
Phagocytes - microbiology
Phagocytosis
Phagocytosis - immunology
Phagosomes - metabolism
Phagosomes - microbiology
Piscidins
Sea Bream - immunology
Sea Bream - metabolism
Sea Bream - microbiology
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Summary:Antimicrobial peptides (AMPs) are increasingly recognized as a critical first line of defence against many pathogens. The genes encoding these peptides are expressed in numerous tissue and cell types from a wide variety of different species including mammals, amphibians, fish, and insects. In this study, we report that the AMPs called piscidins were primarily present in the mast cells (MCs) of fish and were only identified in fish belonging to the Order Perciformes. It is striking that histamine was seen to have a similar evolutionary history, since the only piscine MCs endowed with this molecule are in the Perciformes. We also show that both MCs and professional phagocytic granulocytes were armed with different piscidin molecules. In contrast, macrophages were devoid of these AMPs. More importantly, we found by immunoelectron microscopy that piscidins were delivered to the bacteria-containing phagosome of granulocytes upon phagocytosis, suggesting a role for these AMPs in the killing of both extracellular and intracellular pathogenic bacteria.
ISSN:0145-305X
1879-0089
DOI:10.1016/j.dci.2008.05.015