Distribution of neuromedin U binding sites in the rat CNS revealed by in vitro receptor autoradiography

Abstract Neuromedin U (NMU), a neuropeptide implicated in feeding, inflammation, pain control and anxiety-related behaviours, is widely distributed in peripheral organs and the CNS. These effects are thought to be mediated by its receptors NMU1 and NMU2 . Since its precise sites of interaction in th...

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Bibliographic Details
Published in:Neuropeptides (Edinburgh) 2008-08, Vol.42 (4), p.377-386
Main Authors: Mangold, C, Ksiazek, I, Yun, S.-W, Berger, E, Binkert, C
Format: Article
Language:English
Subjects:
125I-NMU-23
Advanced Basic Science
Animals
Autoradiography
Binding Sites
Biological and medical sciences
Brain
Central Nervous System - anatomy & histology
Central Nervous System - metabolism
Endocrinology & Metabolism
Fundamental and applied biological sciences. Psychology
Humans
Male
Neuropeptides - metabolism
Peptide binding in vitro
Protein Isoforms - metabolism
Rats
Rats, Wistar
Receptor mapping
Receptors, Neurotransmitter - metabolism
Spinal cord
Vertebrates: endocrinology
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Summary:Abstract Neuromedin U (NMU), a neuropeptide implicated in feeding, inflammation, pain control and anxiety-related behaviours, is widely distributed in peripheral organs and the CNS. These effects are thought to be mediated by its receptors NMU1 and NMU2 . Since its precise sites of interaction in the CNS were to date unknown, we studied the distribution of in vitro binding sites for125 I-NMU-23 in the rat CNS by receptor autoradiography. High-density specific binding was found in discrete areas of the brain and spinal cord, namely in the limbic system (hippocampal formation, septohippocampal nucleus, indusium griseum, hypothalamus, amygdaloid nuclei), superior colliculus, dorsal raphé, and substantia gelatinosa of the spinal cord. Our findings provide further supportive evidence for a multifunctional role for the peptide in the brain and spinal cord.
ISSN:0143-4179
1532-2785
DOI:10.1016/j.npep.2008.04.008