Bovine spermatozoal head size variation and evaluation of a separation technique based on this size

The objectives of this study were to determine the variation of head areas of normal spermatozoa attributable to breed, individual bull and ejaculate and to verify separation of X and Y chromosome-bearing spermatozoa and separation effectiveness. Spermatozoa were evaluated using video enhanced contr...

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Bibliographic Details
Published in:Theriogenology 1999-10, Vol.52 (6), p.1021-1034
Main Authors: Chandler, J.E., Wilson, M.P., Canal, A.M., Steinholt-Chenevert, H.
Format: Article
Language:English
Subjects:
Animals
area
bovine
breed differences
bulls
Cattle
Cell Separation - methods
Ejaculation
fluorescence
fluorescent intensity
head area
Male
Microscopy, Video
PCR
Polymerase Chain Reaction
purification
rapid methods
semen separation
Sex Determination Analysis - methods
Sex Determination Analysis - veterinary
Sperm Head - ultrastructure
spermatozoa
Spermatozoa - cytology
X Chromosome
x-y separation
Y Chromosome
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Summary:The objectives of this study were to determine the variation of head areas of normal spermatozoa attributable to breed, individual bull and ejaculate and to verify separation of X and Y chromosome-bearing spermatozoa and separation effectiveness. Spermatozoa were evaluated using video enhanced contrast microscopy combined with video intensified fluorescent microscopy and the polymerase chain reaction (PCR). In Experiment 1, spermatozoal head areas were measured from 2 ejaculates collected from bulls of 3 beef and 2 dairy breeds. No differences in head areas were found between breeds or between bulls within breeds; variation was observed among ejaculates from individual bulls across breeds. In Experiment 2, spermatozoa from 5 ejaculates were separated on individual SEPDEVICEs (Patented). Head area, fluorescent intensity and PCR of spermatozoa retained in the SEPDEVICEs suggested a separation based on size in 1 of 5 samples. Ejaculate variation in head areas affected separation efficiency.
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(99)00190-9