Resuscitation-promoting factors are expressed in Mycobacterium tuberculosis -infected human tissue

Summary Resuscitation-promoting factors are small proteins found in many high G+C Gram-positive organisms. In picomolar concentrations in vitro they promote the emergence from dormancy and shorten the lag phase of growth of small inocula of responsive bacteria. Mycobacterium tuberculosis has five rp...

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Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2008-09, Vol.88 (5), p.462-468
Main Authors: Davies, A.P, Dhillon, A.P, Young, M, Henderson, B, McHugh, T.D, Gillespie, S.H
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - immunology
Bacterial Proteins - metabolism
Chronic Disease
Cytokines - genetics
Cytokines - immunology
Cytokines - metabolism
Gene Expression Regulation, Bacterial - genetics
Humans
Immunocytochemistry
Immunohistochemistry - methods
Infectious Disease
Lymph Nodes - metabolism
Lymph Nodes - microbiology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - metabolism
Pulmonary/Respiratory
Resuscitation promoting factors
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Summary:Summary Resuscitation-promoting factors are small proteins found in many high G+C Gram-positive organisms. In picomolar concentrations in vitro they promote the emergence from dormancy and shorten the lag phase of growth of small inocula of responsive bacteria. Mycobacterium tuberculosis has five rpf gene orthologues all of which are expressed during in vitro  growth. Rpfs may play a role in dormancy or reactivation of tubercle bacilli in vivo, and could be a potential target for chemotherapeutic intervention: however it is not known whether they are expressed by M. tuberculosis in vivo in the context of human infection. The work presented here demonstrates that Rpf expression can be detected in human tissues infected with M. tuberculosis and describes the pattern of this expression using immunocytochemistry with anti-Rpf antibodies.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2008.01.007