The effect of egg yolk, low density lipoproteins, methylxanthines and fertilization diluent on cryopreservation efficiency of northern pike ( Esox lucius) spermatozoa

The effect of egg yolk, low density lipoproteins (LDL) as well as methylxanthines (caffeine and theophylline) and fertilization diluent on cryopreservation efficiency of northern pike, Esox lucius, spermatozoa was tested. Milt was cryopreserved in pellets on dry ice then stored in liquid nitrogen. T...

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Bibliographic Details
Published in:Theriogenology 1999-08, Vol.52 (3), p.473-479
Main Authors: Babiak, I., Glogowski, J., Luczynski, M.J., Luczynski, M., Demianowicz, W.
Format: Article
Language:English
Subjects:
Animals
artificial insemination
buffers
caffeine
cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents
Dimethyl Sulfoxide
Egg Yolk
Esocidae
Female
fertilization
Fertilization in Vitro - methods
Fertilization in Vitro - veterinary
fish
hatching
hatching rate
Lipoproteins, LDL
low density lipoprotein
Male
milt
pike
pike spermatozoa
semen
semen preservation
Semen Preservation - methods
Semen Preservation - veterinary
Sperm-Ovum Interactions
Spermatozoa - cytology
Spermatozoa - physiology
Sucrose
theophylline
Xanthines
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Summary:The effect of egg yolk, low density lipoproteins (LDL) as well as methylxanthines (caffeine and theophylline) and fertilization diluent on cryopreservation efficiency of northern pike, Esox lucius, spermatozoa was tested. Milt was cryopreserved in pellets on dry ice then stored in liquid nitrogen. The extender consisted of 0.6 M sucrose + 15% DMSO supplemented with egg yolk or LDL fractions. The most effective results (77.3% hatched larvae vs 74.1% in the control group) were obtained from extender that contained only 0.6 M sucrose + 15% DMSO and was used for freezing, while the fertilization diluent was used for thawing. Addition of egg yolk or LDL to the extender did not improve the results. The presence of caffeine in the thawing solution significantly lowered fertilization rate of cryopreserved spermatozoa, whereas theophylline did not significantly affect the results. The addition of fertilization diluent to the eggs prior to insemination was superior to the other treatments. The proposed procedure constitutes a complete method for the efficient cryopreservation of northern pike semen.
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(99)00144-2