Two-dimensional polyacrylamide gel electrophoresis of equine seminal plasma proteins and their correlation with fertility

The objectives of this study were to 1) identify proteins found in stallion seminal plasma utilizing two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) in conjunction with Western blot analysis; and 2) to determine if any of these individual proteins were correlated with stallion fertility...

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Bibliographic Details
Published in:Theriogenology 1999-10, Vol.52 (5), p.863-873
Main Authors: Brandon, C.I., Heusner, G.L., Caudle, A.B., Fayrer-Hosken, R.A.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
antifertility
antifertility factors
Biomarkers
Blotting, Western
Cattle
conception rate
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Fertility
Horses
Insemination, Artificial - veterinary
isoelectric focusing
Male
male fertility
molecular weight
osteopontin
polyacrylamide gel electrophoresis
Prostatic Secretory Proteins
protein
proteins
Proteins - analysis
Regression Analysis
Semen - chemistry
seminal plasma
Seminal Plasma Proteins
Stallion
stallions
Western blotting
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Summary:The objectives of this study were to 1) identify proteins found in stallion seminal plasma utilizing two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) in conjunction with Western blot analysis; and 2) to determine if any of these individual proteins were correlated with stallion fertility utilizing regression analysis. Fertility was quantified by assigning a breeding score for each stallion. Each score was calculated by dividing the number of conceptions by the number of breedings for each stallion for four successive breeding seasons (1992–1995). Ejaculates from stallions of known fertility (n=6) were collected with a Missouri-style artificial vagina. Immediately after collection, the semen sample was filtered and the gel fraction removed. The resultant sperm-rich fraction was centrifuged in a Beckman Microfuge E at 10,000 x g and the seminal plasma aspirated from the pelleted sperm cells. Two-dimensional PAGE of the seminal plasma was performed under denaturing conditions which revealed that 14 proteins were common in all stallions in the research population. Four of these proteins (SP-1, SP-2, SP-3, and SP-4) were found to be significantly (P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(99)00178-8