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Automated liquid–liquid extraction based on 96-well plate format in conjunction with ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) for the quantitation of methoxsalen in human plasma
A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid–liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasm...
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| Published in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-09, Vol.872 (1), p.167-171 |
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| cited_by | cdi_FETCH-LOGICAL-c422t-54c9f2409cbd5dd34b6dbc70657c8daa1d2fb644af40f24c060756e2495eedd43 |
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| cites | cdi_FETCH-LOGICAL-c422t-54c9f2409cbd5dd34b6dbc70657c8daa1d2fb644af40f24c060756e2495eedd43 |
| container_end_page | 171 |
| container_issue | 1 |
| container_start_page | 167 |
| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| container_volume | 872 |
| creator | Yadav, Manish Contractor, Pritesh Upadhyay, Vivek Gupta, Ajay Guttikar, Swati Singhal, Puran Goswami, Sailendra Shrivastav, Pranav S. |
| description | A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid–liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasma samples with ketoconazole as internal standard (IS) were prepared by employing 0.2
mL human plasma in ethyl acetate:dichloromethane (80:20, v/v). The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column using isocratic mobile phase, consisting of 10
mM ammonium formate and acetonitrile (60:40, v/v), at a flow rate of 0.5
mL/min. The linear dynamic range was established over the concentration range 1.1–213.1
ng/mL for methoxsalen. The method was rugged and rapid with a total run time of 1.5
min. It was successfully applied to a pivotal bioequivalence study in 12 healthy human subjects after oral administration of 10
mg extended release methoxsalen formulation under fasting condition. |
| doi_str_mv | 10.1016/j.jchromb.2008.06.058 |
| format | article |
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mL human plasma in ethyl acetate:dichloromethane (80:20, v/v). The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column using isocratic mobile phase, consisting of 10
mM ammonium formate and acetonitrile (60:40, v/v), at a flow rate of 0.5
mL/min. The linear dynamic range was established over the concentration range 1.1–213.1
ng/mL for methoxsalen. The method was rugged and rapid with a total run time of 1.5
min. It was successfully applied to a pivotal bioequivalence study in 12 healthy human subjects after oral administration of 10
mg extended release methoxsalen formulation under fasting condition.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2008.06.058</identifier><identifier>PMID: 18656428</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>96-Well plate liquid–liquid extraction ; Analysis ; Analytical, structural and metabolic biochemistry ; Automation ; Biological and medical sciences ; Chromatography, Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; High throughput ; Human plasma ; Humans ; Medical sciences ; Methoxsalen ; Methoxsalen - blood ; Pharmacology. Drug treatments ; Photosensitizing Agents - blood ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Tandem Mass Spectrometry - methods ; UPLC–MS/MS</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2008-09, Vol.872 (1), p.167-171</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-54c9f2409cbd5dd34b6dbc70657c8daa1d2fb644af40f24c060756e2495eedd43</citedby><cites>FETCH-LOGICAL-c422t-54c9f2409cbd5dd34b6dbc70657c8daa1d2fb644af40f24c060756e2495eedd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20667425$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18656428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yadav, Manish</creatorcontrib><creatorcontrib>Contractor, Pritesh</creatorcontrib><creatorcontrib>Upadhyay, Vivek</creatorcontrib><creatorcontrib>Gupta, Ajay</creatorcontrib><creatorcontrib>Guttikar, Swati</creatorcontrib><creatorcontrib>Singhal, Puran</creatorcontrib><creatorcontrib>Goswami, Sailendra</creatorcontrib><creatorcontrib>Shrivastav, Pranav S.</creatorcontrib><title>Automated liquid–liquid extraction based on 96-well plate format in conjunction with ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) for the quantitation of methoxsalen in human plasma</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid–liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasma samples with ketoconazole as internal standard (IS) were prepared by employing 0.2
mL human plasma in ethyl acetate:dichloromethane (80:20, v/v). The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column using isocratic mobile phase, consisting of 10
mM ammonium formate and acetonitrile (60:40, v/v), at a flow rate of 0.5
mL/min. The linear dynamic range was established over the concentration range 1.1–213.1
ng/mL for methoxsalen. The method was rugged and rapid with a total run time of 1.5
min. It was successfully applied to a pivotal bioequivalence study in 12 healthy human subjects after oral administration of 10
mg extended release methoxsalen formulation under fasting condition.</description><subject>96-Well plate liquid–liquid extraction</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Automation</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>High throughput</subject><subject>Human plasma</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methoxsalen</subject><subject>Methoxsalen - blood</subject><subject>Pharmacology. Drug treatments</subject><subject>Photosensitizing Agents - blood</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>UPLC–MS/MS</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkc1u1DAUhSMEoj_wCCBvQLDI1HFsJ7OqqhF_0lQglUrsLMe-IR4lccZ2aGfXd-DtWPIkOJMIlqx8JX_n3qNzkuRFhlcZzvjFbrVTjbNdtSIYlyvMV5iVj5LTrCzyNC_4t8dxZgVOMcnJSXLm_Q7jrMBF_jQ5yUrOOCXlafLragy2kwE0as1-NPr3w895QHAfnFTB2B5V0kcgDmue3kHboqGNElRbF6XI9EjZfjf2M3xnQoPGNorTAdyR6RUs69HRtAz2u5NDc0BB9ho61EnvkR9AhfgLwR3Qm9sv2000c31zcX3zdjqFQgNoP8o-mCCPl2yNItzYey9b6CcfzRiPTe58J58lT2rZeni-vOfJ7ft3Xzcf0-3nD582V9tUUUJCyqha14Titao00zqnFdeVKjBnhSq1lJkmdcUplTXFkVOY44JxIHTNALSm-Xnyet47OLsfwQfRGa9iSrIHO3rB15QXjEwgm0HlrPcOajE400l3EBkWU6liJ5ZSxVSqwFzEUqPu5XJgrDrQ_1RLixF4tQDSK9nWLgZu_F-OYM4LSljkLmcOYhw_DDjhlYFYjjYuRi-0Nf-x8gcfBctf</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Yadav, Manish</creator><creator>Contractor, Pritesh</creator><creator>Upadhyay, Vivek</creator><creator>Gupta, Ajay</creator><creator>Guttikar, Swati</creator><creator>Singhal, Puran</creator><creator>Goswami, Sailendra</creator><creator>Shrivastav, Pranav S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Automated liquid–liquid extraction based on 96-well plate format in conjunction with ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) for the quantitation of methoxsalen in human plasma</title><author>Yadav, Manish ; Contractor, Pritesh ; Upadhyay, Vivek ; Gupta, Ajay ; Guttikar, Swati ; Singhal, Puran ; Goswami, Sailendra ; Shrivastav, Pranav S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-54c9f2409cbd5dd34b6dbc70657c8daa1d2fb644af40f24c060756e2495eedd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>96-Well plate liquid–liquid extraction</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Automation</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>High throughput</topic><topic>Human plasma</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methoxsalen</topic><topic>Methoxsalen - blood</topic><topic>Pharmacology. Drug treatments</topic><topic>Photosensitizing Agents - blood</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>UPLC–MS/MS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yadav, Manish</creatorcontrib><creatorcontrib>Contractor, Pritesh</creatorcontrib><creatorcontrib>Upadhyay, Vivek</creatorcontrib><creatorcontrib>Gupta, Ajay</creatorcontrib><creatorcontrib>Guttikar, Swati</creatorcontrib><creatorcontrib>Singhal, Puran</creatorcontrib><creatorcontrib>Goswami, Sailendra</creatorcontrib><creatorcontrib>Shrivastav, Pranav S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>872</volume><issue>1</issue><spage>167</spage><epage>171</epage><pages>167-171</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A sensitive, specific and high throughput bioanalytical method using automated sample processing via 96-well plate liquid–liquid extraction and ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) has been developed for the determination of methoxsalen in human plasma. Plasma samples with ketoconazole as internal standard (IS) were prepared by employing 0.2
mL human plasma in ethyl acetate:dichloromethane (80:20, v/v). The chromatographic separation was achieved on a Waters Acquity UPLC BEH C18 column using isocratic mobile phase, consisting of 10
mM ammonium formate and acetonitrile (60:40, v/v), at a flow rate of 0.5
mL/min. The linear dynamic range was established over the concentration range 1.1–213.1
ng/mL for methoxsalen. The method was rugged and rapid with a total run time of 1.5
min. It was successfully applied to a pivotal bioequivalence study in 12 healthy human subjects after oral administration of 10
mg extended release methoxsalen formulation under fasting condition.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18656428</pmid><doi>10.1016/j.jchromb.2008.06.058</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2008-09, Vol.872 (1), p.167-171 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69467524 |
| source | ScienceDirect Freedom Collection |
| subjects | 96-Well plate liquid–liquid extraction Analysis Analytical, structural and metabolic biochemistry Automation Biological and medical sciences Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology High throughput Human plasma Humans Medical sciences Methoxsalen Methoxsalen - blood Pharmacology. Drug treatments Photosensitizing Agents - blood Reference Standards Reproducibility of Results Sensitivity and Specificity Tandem Mass Spectrometry - methods UPLC–MS/MS |
| title | Automated liquid–liquid extraction based on 96-well plate format in conjunction with ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) for the quantitation of methoxsalen in human plasma |
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