Culture shock. Molecular methods for diagnosis of infectious diseases

Nucleic acid detection methods, such as polymerase chain reaction (PCR), can often detect specific microbial pathogens, virulence markers and antimicrobial resistance genes more rapidly and with greater sensitivity and specificity than culture and conventional identification and susceptibility testi...

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Bibliographic Details
Published in:Medical journal of Australia 1999-11, Vol.171 (10), p.536-540
Main Authors: Gilbert, G L, James, G S, Sintchenko, V
Format: Article
Language:English
Subjects:
DNA Primers
Drug Resistance, Microbial - genetics
Genetic Techniques
Humans
Infection - diagnosis
Infection - genetics
Microbiological Techniques
Nucleic Acid Hybridization - methods
Nucleic Acid Probes
Nucleic Acids - analysis
Polymerase Chain Reaction
Sensitivity and Specificity
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Summary:Nucleic acid detection methods, such as polymerase chain reaction (PCR), can often detect specific microbial pathogens, virulence markers and antimicrobial resistance genes more rapidly and with greater sensitivity and specificity than culture and conventional identification and susceptibility testing. Multiplex PCR can detect multiple genes in a single assay; this capability will be greatly extended by new techniques such as the DNA chip. However, limitations and pitfalls of nucleic detection methods remain.
ISSN:0025-729X
1326-5377
DOI:10.5694/j.1326-5377.1999.tb123789.x