The Synthesis and Activity of Lipoprotein Lipase in the Subcutaneous Adipose Tissue of Patients with Musculoskeletal Sarcomas

The purpose of this study was to explore the triacylglycerol (TG) deposition and lipoprotein lipase (LPL) activity in the adipose tissue of patients with muculoskeletal sarcoma. Subcutaneous adipose tissue was obtained from the thighs of 19 patients with musculoskeletal sarcomas (sarcoma group) and...

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Bibliographic Details
Published in:Anticancer research 2008-07, Vol.28 (4B), p.2081-2086
Main Authors: SAKAYAMA, Kenshi, KIDANI, Teruki, TANJI, Nozomu, YAMAMOTO, Haruyasu, MASUNO, Hiroshi
Format: Article
Language:English
Subjects:
Adipose Tissue - enzymology
Adipose Tissue - metabolism
Adolescent
Adult
Aged
Biological and medical sciences
Body Mass Index
Bone Neoplasms - enzymology
Bone Neoplasms - metabolism
Female
Humans
Lipoprotein Lipase - biosynthesis
Lipoprotein Lipase - metabolism
Male
Medical sciences
Middle Aged
Muscle Neoplasms - enzymology
Muscle Neoplasms - metabolism
Sarcoma - enzymology
Sarcoma - metabolism
Triglycerides - metabolism
Tumors
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Summary:The purpose of this study was to explore the triacylglycerol (TG) deposition and lipoprotein lipase (LPL) activity in the adipose tissue of patients with muculoskeletal sarcoma. Subcutaneous adipose tissue was obtained from the thighs of 19 patients with musculoskeletal sarcomas (sarcoma group) and 20 patients with osteoarthritis of the hip joint (control group) at surgery. The adipose tissue was homogenized and aliquots of the homogenate were used to measure the TG content and to prepare an acetone/ether powder to measure the LPL activity. The TG content was higher, but not significantly, in the sarcoma group than in the control group. The LPL activity of the sarcoma group was significantly higher than that of the control group. The TG content of the sarcoma group correlated positively with the LPL activity. [ 35 S]Methionine incorporation investigation showed that the rate of LPL synthesis was significantly higher in the sarcoma group than in the control group. These results indicated that LPL was up-regulated at the transcriptional/translational level, thus resulting in an increased TG deposition in the adipose tissue of patients with muculoskeletal sarcoma.
ISSN:0250-7005
1791-7530