Genetic polymorphism of the kappa-casein gene in Brazilian cattle

Frequencies of kappa-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Gyr cows, Guzerat sires, Guzerat cows, Nellore sires, and Gyr x Holstein crossbreds) by means of polymerase chain reaction-restriction fragment length polym...

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Bibliographic Details
Published in:Genetics and molecular research 2008-01, Vol.7 (3), p.623-630
Main Authors: Azevedo, A.L.S, Nascimento, C.S, Steinberg, R.S, Carvalho, M.R.S, Peixoto, M.G.C.D, Teodoro, R.L, Verneque, R.S, Guimarães, S.E.F, Machado, M.A
Format: Article
Language:English
Subjects:
alleles
Animals
Bos indicus
Breeding
Caseins - genetics
cattle
Cattle - genetics
Cheese
Gene Frequency
genetic polymorphism
genetic variation
kappa-casein
Polymerase Chain Reaction
Polymorphism, Genetic
restriction fragment length polymorphism
Species Specificity
zebu
zebu breeds
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Summary:Frequencies of kappa-casein gene alleles were determined in 1316 animals from the Brazilian Bos indicus genetic groups (Sindhi cows, Gyr sires, Gyr cows, Guzerat sires, Guzerat cows, Nellore sires, and Gyr x Holstein crossbreds) by means of polymerase chain reaction-restriction fragment length polymorphism analysis using two independent restriction nucleases (Hinf I and HaeIII). The genotyping of kappa-casein alleles (A and B) is of practical importance, since the B allele is found to correlate with commercially valuable parameters of cheese yielding efficiency. The frequencies of the B allele of kappa-casein among breeds ranged from 0.01 to 0.30. The Sindhi breed had the highest frequency for the B allele (0.30), while the frequencies of this allele in other breeds ranged from 0.01 to 0.18. A wide variation in the B allele frequency among B. indicus breeds was found suggesting that molecular selection for animals carrying the B allele could impact breeding programs for dairy production.
ISSN:1676-5680
1676-5680
DOI:10.4238/vol7-3gmr428