Production of Organically Bound Selenium Yeast by Continuous Fermentation

This paper describes a protocol for incorporation of sodium selenite or sodium selenate into Saccharomyces cerevisiae biomass by continuous fermentation in a medium with minimal sulfur and methionine concentrations. Selenium incorporation was followed by atomic absorption analysis and methylene blue...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-06, Vol.47 (6), p.2491-2495
Main Authors: Demirci, Ali, Pometto, Anthony L
Format: Article
Language:English
Subjects:
Arsenicals - metabolism
Biological and medical sciences
biomass
Biotechnology
continuous fermentation
culture media
Fermentation
Food industries
Fundamental and applied biological sciences. Psychology
inorganic salts
ion transport
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
mineral content
nutrient availability
nutrient content
nutrient sources
organic compounds
organoselenium compounds
Research and development. New food products, dietetic foods and beverages
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Selenic Acid
selenium
Selenium Compounds - metabolism
sodium selenate
sodium selenite
Sodium Selenite - metabolism
sodium sulfate
sulfur
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Summary:This paper describes a protocol for incorporation of sodium selenite or sodium selenate into Saccharomyces cerevisiae biomass by continuous fermentation in a medium with minimal sulfur and methionine concentrations. Selenium incorporation was followed by atomic absorption analysis and methylene blue reduction time (MBRT). Continuous fermentation at 0.2 h-1 dilution rate and sodium selenite addition gradient up to 0.69 g/L of Na2SeO3 yielded 1.89 g/L of biomass with 1904 μg of selenium/g of dry biomass. However, MBRT was 0.1 min, which indicated that the majority of selenium was in the inorganic form. On the other hand, continuous fermentation at 0.2 h-1 dilution rate and sodium selenate gradient up to 0.28 g/L of Na2SeO4 yielded 0.76 g/L of dry biomass with 687 μg of selenium/g of dry biomass, and MBRT was 26 min, which indicated a high concentration of organically bound selenium. Overall, the results indicate a Se/S ratio of 3.9:1 and a dry biomass/Se ratio of 5.5:1 as optimal for continuous production of organically bound selenium. Keywords: Selenium; yeast; Saccharomyces cerevisiae; fermentation
ISSN:0021-8561
1520-5118
DOI:10.1021/jf981198y