2,2-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components from Polygonum multiflorum Thunb

An activity-directed fractionation and purification process was used to identify the antioxidative components of Polygonum multiflorum Thunb. (PM). Dried root of PM was extracted with 95% ethanol and then separated into water, ethyl acetate, and hexane fractions. Among these only the ethyl acetate p...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1999-06, Vol.47 (6), p.2226-2228
Main Authors: Chen, Yong, Wang, Mingfu, Rosen, Robert T, Ho, Chi-Tang
Format: Article
Language:English
Subjects:
2,3,5,4'-tetrahydroxystilbene 2-o-beta-d-glucopyranoside
Agronomy. Soil science and plant productions
Antioxidants - chemistry
Antioxidants - isolation & purification
Bepridil - analogs & derivatives
Bepridil - chemistry
Bepridil - isolation & purification
Biological and medical sciences
Biphenyl Compounds
catechin
Chemical constitution
chemical reactions
chemical structure
Economic plant physiology
Free Radical Scavengers - chemistry
Free Radical Scavengers - isolation & purification
Free Radicals
Fundamental and applied biological sciences. Psychology
gallic acid
General pharmacology
glucosides
Medical sciences
medicinal plants
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
phenolic compounds
Picrates
plant extracts
Plant Roots
Plants, Medicinal - chemistry
Polygonum
roots
stilbenes
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Summary:An activity-directed fractionation and purification process was used to identify the antioxidative components of Polygonum multiflorum Thunb. (PM). Dried root of PM was extracted with 95% ethanol and then separated into water, ethyl acetate, and hexane fractions. Among these only the ethyl acetate phase showed strong antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) test when compared with water and hexane phases. The ethyl acetate fraction was then subjected to separation and purification using silica gel column chromatography and Sephadex LH-20 chromatography. Three compounds showing strong antioxidant activity were identified by spectral methods (1H NMR, 13C NMR, and MS) and by comparison with authentic samples to be gallic acid, catechin, and 2,3,5,4‘-tetrahydroxystilbene 2-O-β-d-glucopyranoside. Keywords: Polygonum multiflorum Thunb.; free radical; antioxidant activity; 2,3,5,4‘-tetrahydroxystilbene 2-O-β-d-glucopyranoside
ISSN:0021-8561
1520-5118
DOI:10.1021/jf990092f