Expression and tyrosine phosphorylation of Crk-associated substrate lymphocyte type (Cas-L) protein in human neutrophils

Crk‐associated substrate lymphocyte type (Cas‐L) protein, also known as human enhancer of filamentation 1 (Hef1) or neural precursor cell‐expressed, developmentally down‐regulated gene 9 (Nedd9), belongs to the Cas family of adapter proteins, which are involved in integrin signaling. Previous report...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2008-09, Vol.105 (1), p.121-128
Main Authors: Nakamoto, Tetsuya, Seo, Sachiko, Sakai, Ryuichi, Kato, Takayuki, Kutsuna, Haruo, Kurokawa, Mineo, Noda, Masaki, Miyasaka, Nobuyuki, Kitagawa, Seiichi
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - metabolism
B-cell receptor
Cas-L
Cell Adhesion
Cell Line
fMLP
Hef1
Humans
lipopolysaccharide
Lipopolysaccharides - pharmacology
Nedd9
neutrophil
Neutrophils - cytology
Neutrophils - drug effects
Neutrophils - metabolism
Phosphoproteins - metabolism
Phosphotyrosine - metabolism
tumor necrosis factor alpha
Tumor Necrosis Factor-alpha - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Crk‐associated substrate lymphocyte type (Cas‐L) protein, also known as human enhancer of filamentation 1 (Hef1) or neural precursor cell‐expressed, developmentally down‐regulated gene 9 (Nedd9), belongs to the Cas family of adapter proteins, which are involved in integrin signaling. Previous reports showed that Cas‐L is expressed preferentially in lymphocytes and epithelial cells. Cas‐L mediates signals from integrins, T‐cell receptors, B‐cells receptors, and transforming growth factor beta, leading to cell movement and cell division. Here, we report the expression of Cas‐L in neutrophils. Cas‐L was tyrosine‐phosphorylated when human neutrophils were stimulated by fMLP, tumor necrosis factor‐alpha (TNF), or lipopolysaccharide. The tyrosine phosphorylation of Cas‐L in fMLP‐ or TNF‐ stimulated neutrophils was further enhanced by adhesion of the cells to their substrates. Cas‐L was found to be localized at focal adhesions in stimulated neutrophils based on immunofluorescence microscopy. These findings suggest that Cas‐L is one of the targets of inflammatory cytokines and is also modulated by cell adhesion process in neutrophils. J. Cell. Biochem. 105: 121–128, 2008. © 2008 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.21799