A paper-based electroelution system for protein recovery from stained sodium dodecyl sulfate–polyacrylamide gels

Electroelution is a widely used methodology for protein purification. In this study, a practical and low-cost system for protein electroelution from stained polyacrylamide gels was developed. For this, a horizontal protein electroelution cuve was constructed with glass plates, 1.5-ml capacity microc...

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Bibliographic Details
Published in:Analytical biochemistry 2008-10, Vol.381 (2), p.267-269
Main Authors: Branco, Alan T., Ferreira, Beatriz dos S., de Souza Filho, Gonçalo A.
Format: Article
Language:English
Subjects:
Electrophoresis, Polyacrylamide Gel
Proteins - isolation & purification
Sodium Dodecyl Sulfate - chemistry
Staining and Labeling
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Summary:Electroelution is a widely used methodology for protein purification. In this study, a practical and low-cost system for protein electroelution from stained polyacrylamide gels was developed. For this, a horizontal protein electroelution cuve was constructed with glass plates, 1.5-ml capacity microcentrifuge tubes, and dialysis membrane. Analyses of the system efficiency showed high protein recovery from nonfixed and fixed sodium dodecyl sulfate (SDS)–polyacrylamide gels.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2008.03.025