Two Mass-Spectrometric Techniques for Quantifying Serine Enantiomers and Glycine in Cerebrospinal Fluid: Potential Confounders and Age-Dependent Ranges

The recent discovery and specific functions of D-amino acids in humans are bound to lead to the revelation of D-amino acid abnormalities in human disorders. Therefore, high-throughput analysis techniques are warranted to determine D-amino acids in biological fluids in a routine laboratory setting. W...

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Published in:Clinical chemistry (Baltimore, Md.) Md.), 2008-09, Vol.54 (9), p.1443-1450
Main Authors: Fuchs, Sabine A, de Sain-van der Velden, Monique G. M, de Barse, Martina M. J, Roeleveld, Martin W, Hendriks, Margriet, Dorland, Lambertus, Klomp, Leo W. J, Berger, Ruud, de Koning, Tom J
Format: Article
Language:English
Subjects:
Adolescent
Adult
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cerebrospinal Fluid - chemistry
Child
Child, Preschool
Fundamental and applied biological sciences. Psychology
Glycine - chemistry
Health
Humans
Infant
Infant, Newborn
Investigative techniques, diagnostic techniques (general aspects)
Mass Spectrometry - methods
Medical sciences
Serine - chemistry
Stereoisomerism
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Summary:The recent discovery and specific functions of D-amino acids in humans are bound to lead to the revelation of D-amino acid abnormalities in human disorders. Therefore, high-throughput analysis techniques are warranted to determine D-amino acids in biological fluids in a routine laboratory setting. We developed 2 chromatographic techniques, a nonchiral derivatization with chiral (chirasil-L-val column) separation in a GC-MS system and a chiral derivatization with Marfey's reagent and LC- MS analysis. We validated the techniques for D-serine, L-serine, and glycine determination in cerebrospinal fluid (CSF), evaluated several confounders, and determined age-dependent human concentration ranges. Quantification limits for D-serine, L-serine, and glycine in cerebrospinal fluid were 0.14, 0.44, and 0.14 micromol/L, respectively, for GC-MS and 0.20, 0.41, and 0.14 micromol/L for LC-MS. Within-run imprecision was
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2007.100412