Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases

The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially ava...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2008-09, Vol.54 (9), p.1546-1553
Main Authors: Kraenzlin, Marius E, Kraenzlin, Claude A, Meier, Christian, Giunta, Cecilia, Steinmann, Beat
Format: Article
Language:English
Subjects:
Adult
Age Distribution
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Bone density
Bone diseases
Bone Diseases, Metabolic - urine
Bones
Chromatography, High Pressure Liquid - methods
Circadian rhythm
Collagen - urine
Disease
Female
Fluorescence
Fundamental and applied biological sciences. Psychology
Genetic disorders
Health
Humans
Hydrochloric acid
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Medical sciences
Menopause - urine
Metabolic disorders
Middle Aged
Osteoporosis
Womens health
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Summary:The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially available reagent set (Chromsystems Instruments & Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10). The mean intraassay and interassay CVs were
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2008.105262