Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases

The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially ava...

Full description

Saved in:
Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2008-09, Vol.54 (9), p.1546-1553
Main Authors: Kraenzlin, Marius E, Kraenzlin, Claude A, Meier, Christian, Giunta, Cecilia, Steinmann, Beat
Format: Article
Language:English
Subjects:
Adult
Age Distribution
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Bone density
Bone diseases
Bone Diseases, Metabolic - urine
Bones
Chromatography, High Pressure Liquid - methods
Circadian rhythm
Collagen - urine
Disease
Female
Fluorescence
Fundamental and applied biological sciences. Psychology
Genetic disorders
Health
Humans
Hydrochloric acid
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Medical sciences
Menopause - urine
Metabolic disorders
Middle Aged
Osteoporosis
Womens health
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73
cites cdi_FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73
container_end_page 1553
container_issue 9
container_start_page 1546
container_title Clinical chemistry (Baltimore, Md.)
container_volume 54
creator Kraenzlin, Marius E
Kraenzlin, Claude A
Meier, Christian
Giunta, Cecilia
Steinmann, Beat
description The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially available reagent set (Chromsystems Instruments & Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10). The mean intraassay and interassay CVs were
doi_str_mv 10.1373/clinchem.2008.105262
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69484633</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69484633</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73</originalsourceid><addsrcrecordid>eNpdkV1v0zAYhS0EYmXwDxCykOBqKf5I_MFdyQab1Aku2LX1xrHbjCQudqJq_36uWpi0K_uVnvfY5xyE3lOypFzyL7bvRrt1w5IRopaUVEywF2hBK04KVQn6Ei0IIbrQtJRn6E1K93kspRKv0RlVouKKiQXqV_MUBphci69_rWu8SgkesA8R38VuhPiA69D3sHEjrmNIqciv_klf8ZX3zk44eLzauAt868awgznlK4xtHidoQt9Z_C2MDl92yUFy6S165aFP7t3pPEd3369-19fF-uePm3q1LmzJ9VQ0vgFlCanaVmpuJWG0pR4cEK-YL6VWjlIteMtLoFo1zDIlSAUy22s4SH6OPh91dzH8nV2azNAl67KP0YU5GaFLVQrOM_jxGXgf5jjmvxlGuVaCSZqh8gjZQwDRebOL3ZCjMZSYQxXmXxXmUIU5VpHXPpy052Zw7dPSKfsMfDoBkCz0PsJou_SfYySLSEGe3Gy7zXbfRWfSAH2fZanZ7_dVabShVfbzCG1qny8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>213986271</pqid></control><display><type>article</type><title>Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases</title><source>Oxford Journals Online</source><creator>Kraenzlin, Marius E ; Kraenzlin, Claude A ; Meier, Christian ; Giunta, Cecilia ; Steinmann, Beat</creator><creatorcontrib>Kraenzlin, Marius E ; Kraenzlin, Claude A ; Meier, Christian ; Giunta, Cecilia ; Steinmann, Beat</creatorcontrib><description>The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially available reagent set (Chromsystems Instruments &amp; Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10). The mean intraassay and interassay CVs were &lt;6% and &lt;8% for both PYD and DPD, respectively. The reference interval was constant for premenopausal women in the age group 20-49 years. In men, cross-link values peaked at 20-29 years and decreased thereafter. Women with postmenopausal osteoporosis had significantly higher PYD (51%) and DPD (58%) values compared to premenopausal women. Similar results were found in osteoporotic men. In children the highest values were found in the first weeks and months after birth, followed by a decrease of 50%-60% at age 11-14 years. In metabolic bone diseases cross-link concentrations were significantly increased. The DPD:PYD ratio (mean value approximately 0.2) was remarkably constant in all populations evaluated. The automated HPLC assay is a precise and convenient method for PYD and DPD measurement. We established reference intervals for adult women and men and for children up to 14 years old. The cross-link concentrations we determined by use of this HPLC method confirm its clinical value in enabling identification of increased bone resorption in patients with metabolic bone diseases.</description><identifier>ISSN: 0009-9147</identifier><identifier>EISSN: 1530-8561</identifier><identifier>DOI: 10.1373/clinchem.2008.105262</identifier><identifier>PMID: 18653826</identifier><identifier>CODEN: CLCHAU</identifier><language>eng</language><publisher>Washington, DC: Am Assoc Clin Chem</publisher><subject>Adult ; Age Distribution ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Bone density ; Bone diseases ; Bone Diseases, Metabolic - urine ; Bones ; Chromatography, High Pressure Liquid - methods ; Circadian rhythm ; Collagen - urine ; Disease ; Female ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Genetic disorders ; Health ; Humans ; Hydrochloric acid ; Investigative techniques, diagnostic techniques (general aspects) ; Liquid chromatography ; Male ; Medical sciences ; Menopause - urine ; Metabolic disorders ; Middle Aged ; Osteoporosis ; Womens health</subject><ispartof>Clinical chemistry (Baltimore, Md.), 2008-09, Vol.54 (9), p.1546-1553</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Association for Clinical Chemistry Sep 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73</citedby><cites>FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=20623760$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18653826$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kraenzlin, Marius E</creatorcontrib><creatorcontrib>Kraenzlin, Claude A</creatorcontrib><creatorcontrib>Meier, Christian</creatorcontrib><creatorcontrib>Giunta, Cecilia</creatorcontrib><creatorcontrib>Steinmann, Beat</creatorcontrib><title>Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases</title><title>Clinical chemistry (Baltimore, Md.)</title><addtitle>Clin Chem</addtitle><description>The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially available reagent set (Chromsystems Instruments &amp; Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10). The mean intraassay and interassay CVs were &lt;6% and &lt;8% for both PYD and DPD, respectively. The reference interval was constant for premenopausal women in the age group 20-49 years. In men, cross-link values peaked at 20-29 years and decreased thereafter. Women with postmenopausal osteoporosis had significantly higher PYD (51%) and DPD (58%) values compared to premenopausal women. Similar results were found in osteoporotic men. In children the highest values were found in the first weeks and months after birth, followed by a decrease of 50%-60% at age 11-14 years. In metabolic bone diseases cross-link concentrations were significantly increased. The DPD:PYD ratio (mean value approximately 0.2) was remarkably constant in all populations evaluated. The automated HPLC assay is a precise and convenient method for PYD and DPD measurement. We established reference intervals for adult women and men and for children up to 14 years old. The cross-link concentrations we determined by use of this HPLC method confirm its clinical value in enabling identification of increased bone resorption in patients with metabolic bone diseases.</description><subject>Adult</subject><subject>Age Distribution</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Bone density</subject><subject>Bone diseases</subject><subject>Bone Diseases, Metabolic - urine</subject><subject>Bones</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Circadian rhythm</subject><subject>Collagen - urine</subject><subject>Disease</subject><subject>Female</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic disorders</subject><subject>Health</subject><subject>Humans</subject><subject>Hydrochloric acid</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Liquid chromatography</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Menopause - urine</subject><subject>Metabolic disorders</subject><subject>Middle Aged</subject><subject>Osteoporosis</subject><subject>Womens health</subject><issn>0009-9147</issn><issn>1530-8561</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpdkV1v0zAYhS0EYmXwDxCykOBqKf5I_MFdyQab1Aku2LX1xrHbjCQudqJq_36uWpi0K_uVnvfY5xyE3lOypFzyL7bvRrt1w5IRopaUVEywF2hBK04KVQn6Ei0IIbrQtJRn6E1K93kspRKv0RlVouKKiQXqV_MUBphci69_rWu8SgkesA8R38VuhPiA69D3sHEjrmNIqciv_klf8ZX3zk44eLzauAt868awgznlK4xtHidoQt9Z_C2MDl92yUFy6S165aFP7t3pPEd3369-19fF-uePm3q1LmzJ9VQ0vgFlCanaVmpuJWG0pR4cEK-YL6VWjlIteMtLoFo1zDIlSAUy22s4SH6OPh91dzH8nV2azNAl67KP0YU5GaFLVQrOM_jxGXgf5jjmvxlGuVaCSZqh8gjZQwDRebOL3ZCjMZSYQxXmXxXmUIU5VpHXPpy052Zw7dPSKfsMfDoBkCz0PsJou_SfYySLSEGe3Gy7zXbfRWfSAH2fZanZ7_dVabShVfbzCG1qny8</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Kraenzlin, Marius E</creator><creator>Kraenzlin, Claude A</creator><creator>Meier, Christian</creator><creator>Giunta, Cecilia</creator><creator>Steinmann, Beat</creator><general>Am Assoc Clin Chem</general><general>American Association for Clinical Chemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4U-</scope><scope>7QO</scope><scope>7RV</scope><scope>7TM</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases</title><author>Kraenzlin, Marius E ; Kraenzlin, Claude A ; Meier, Christian ; Giunta, Cecilia ; Steinmann, Beat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Age Distribution</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Bone density</topic><topic>Bone diseases</topic><topic>Bone Diseases, Metabolic - urine</topic><topic>Bones</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Circadian rhythm</topic><topic>Collagen - urine</topic><topic>Disease</topic><topic>Female</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic disorders</topic><topic>Health</topic><topic>Humans</topic><topic>Hydrochloric acid</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Liquid chromatography</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Menopause - urine</topic><topic>Metabolic disorders</topic><topic>Middle Aged</topic><topic>Osteoporosis</topic><topic>Womens health</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kraenzlin, Marius E</creatorcontrib><creatorcontrib>Kraenzlin, Claude A</creatorcontrib><creatorcontrib>Meier, Christian</creatorcontrib><creatorcontrib>Giunta, Cecilia</creatorcontrib><creatorcontrib>Steinmann, Beat</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>University Readers</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing &amp; Allied Health Database</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database (ProQuest Medical &amp; Health Databases)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraenzlin, Marius E</au><au>Kraenzlin, Claude A</au><au>Meier, Christian</au><au>Giunta, Cecilia</au><au>Steinmann, Beat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases</atitle><jtitle>Clinical chemistry (Baltimore, Md.)</jtitle><addtitle>Clin Chem</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>54</volume><issue>9</issue><spage>1546</spage><epage>1553</epage><pages>1546-1553</pages><issn>0009-9147</issn><eissn>1530-8561</eissn><coden>CLCHAU</coden><abstract>The pyridinium cross-links pyridinoline (PYD) and deoxypyridinoline (DPD) are established markers of bone resorption. We evaluated the analytical and clinical performance of a commercially available PYD HPLC assay and established reference intervals in children and adults. We used a commercially available reagent set (Chromsystems Instruments &amp; Chemicals) to measure PYD and DPD in 319 healthy controls (156 premenopausal women, 80 healthy men, and 83 healthy children age 1 month to 14 years) and 397 patients with metabolic bone diseases (postmenopausal osteoporosis, n = 175; male osteoporosis, n = 176; hyperparathyroidism, n = 17; hyperthyroidism, n = 19; Paget disease, n = 10). The mean intraassay and interassay CVs were &lt;6% and &lt;8% for both PYD and DPD, respectively. The reference interval was constant for premenopausal women in the age group 20-49 years. In men, cross-link values peaked at 20-29 years and decreased thereafter. Women with postmenopausal osteoporosis had significantly higher PYD (51%) and DPD (58%) values compared to premenopausal women. Similar results were found in osteoporotic men. In children the highest values were found in the first weeks and months after birth, followed by a decrease of 50%-60% at age 11-14 years. In metabolic bone diseases cross-link concentrations were significantly increased. The DPD:PYD ratio (mean value approximately 0.2) was remarkably constant in all populations evaluated. The automated HPLC assay is a precise and convenient method for PYD and DPD measurement. We established reference intervals for adult women and men and for children up to 14 years old. The cross-link concentrations we determined by use of this HPLC method confirm its clinical value in enabling identification of increased bone resorption in patients with metabolic bone diseases.</abstract><cop>Washington, DC</cop><pub>Am Assoc Clin Chem</pub><pmid>18653826</pmid><doi>10.1373/clinchem.2008.105262</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0009-9147
ispartof Clinical chemistry (Baltimore, Md.), 2008-09, Vol.54 (9), p.1546-1553
issn 0009-9147
1530-8561
language eng
recordid cdi_proquest_miscellaneous_69484633
source Oxford Journals Online
subjects Adult
Age Distribution
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Bone density
Bone diseases
Bone Diseases, Metabolic - urine
Bones
Chromatography, High Pressure Liquid - methods
Circadian rhythm
Collagen - urine
Disease
Female
Fluorescence
Fundamental and applied biological sciences. Psychology
Genetic disorders
Health
Humans
Hydrochloric acid
Investigative techniques, diagnostic techniques (general aspects)
Liquid chromatography
Male
Medical sciences
Menopause - urine
Metabolic disorders
Middle Aged
Osteoporosis
Womens health
title Automated HPLC Assay for Urinary Collagen Cross-links: Effect of Age, Menopause, and Metabolic Bone Diseases
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T13%3A56%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Automated%20HPLC%20Assay%20for%20Urinary%20Collagen%20Cross-links:%20Effect%20of%20Age,%20Menopause,%20and%20Metabolic%20Bone%20Diseases&rft.jtitle=Clinical%20chemistry%20(Baltimore,%20Md.)&rft.au=Kraenzlin,%20Marius%20E&rft.date=2008-09-01&rft.volume=54&rft.issue=9&rft.spage=1546&rft.epage=1553&rft.pages=1546-1553&rft.issn=0009-9147&rft.eissn=1530-8561&rft.coden=CLCHAU&rft_id=info:doi/10.1373/clinchem.2008.105262&rft_dat=%3Cproquest_cross%3E69484633%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c439t-bfba8c005dd793c7021d1faea0f82f4798e11963d34a198b2c28605a7047b3a73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=213986271&rft_id=info:pmid/18653826&rfr_iscdi=true