A new bioreactor for the controlled application of complex mechanical stimuli for cartilage tissue engineering

Mechanical stimuli have been shown to enhance chondrogenesis on both animal and human chondrocytes cultured in vitro. Different mechanical stimuli act simultaneously in vivo in cartilage tissue and their effects have been extensively studied in vitro, although often in a separated manner. A new bior...

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Bibliographic Details
Published in:Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine Journal of engineering in medicine, 2008-07, Vol.222 (5), p.705-715
Main Authors: Laganà, K, Moretti, M, Dubini, G, Raimondi, M T
Format: Article
Language:English
Subjects:
Animals
Bioreactors
Cartilage
Cartilage, Articular - cytology
Cartilage, Articular - growth & development
Cattle
Cell culture
Cell Culture Techniques - instrumentation
Cell Proliferation
Cells, Cultured
Chondrocytes
Chondrocytes - cytology
Chondrocytes - physiology
Chondrogenesis
Construction engineering
Equipment Design
Equipment Failure Analysis
Hydrostatic pressure
In vitro methods and tests
In vivo methods and tests
Mechanical stimuli
Mechanotransduction, Cellular - physiology
Physical Stimulation - methods
Pressure
Pressurization
Protease inhibitors
Scaffolds
Shear stress
Stimuli
Tissue culture
Tissue engineering
Tissue Engineering - instrumentation
Vibration
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Summary:Mechanical stimuli have been shown to enhance chondrogenesis on both animal and human chondrocytes cultured in vitro. Different mechanical stimuli act simultaneously in vivo in cartilage tissue and their effects have been extensively studied in vitro, although often in a separated manner. A new bioreactor is described where different mechanical stimuli, i.e. shear stress and hydrostatic pressure, can be combined in different ways to study the mechanobiology of tissue engineered cartilage. Shear stress is imposed on cells by forcing the culture medium through the scaffolds, whereas a high hydrostatic pressure up to 15 MPa is generated by pressurizing the culture medium. Fluid-dynamic experimental tests have been performed and successful validation of the bioreactor has been carried out by dynamic culture of tissue-engineered cartilage constructs. The bioreactor system allows the investigation of the combined effects of different mechanical stimuli on the development of engineered cartilage, as well as other possible three-dimensional tissue-engineered constructs.
ISSN:0954-4119
2041-3033
DOI:10.1243/09544119JEIM383