Lipolytic and esterolytic activity-based profiling of murine liver

In lipid metabolism, the liver acts as a buffer for transient energy fluctuations. It temporarily stores fatty acids as triacylglycerol and secretes them as very low density lipoprotein into the circulation when the period of maximum lipid load has passed. The lipolytic enzymes responsible for mobil...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2008-09, Vol.8 (17), p.3645-3656
Main Authors: Birner-Gruenberger, Ruth, Susani-Etzerodt, Heidrun, Kollroser, Manfred, Rechberger, Gerald N, Hermetter, Albin
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Azoles
Biological and medical sciences
Biotinylation
Capture agents
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Esterases - metabolism
Fluorescent Dyes
Functional proteomics
Fundamental and applied biological sciences. Psychology
Lipolysis - physiology
Liver - metabolism
Liver proteins
Mice
Miscellaneous
Mouse
Nitrobenzenes
Proteins
Proteome - analysis
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Summary:In lipid metabolism, the liver acts as a buffer for transient energy fluctuations. It temporarily stores fatty acids as triacylglycerol and secretes them as very low density lipoprotein into the circulation when the period of maximum lipid load has passed. The lipolytic enzymes responsible for mobilization of internal lipid stores in the liver have not been identified yet. We introduced active site-directed chemical probes for lipolytic activity profiling in complex mixtures, known as activity-based proteomics, and employed it for global analysis and functional annotation of lipolytic proteins in mouse adipose tissue. Here we report the combined application of two approaches using fluorescent and biotinylated probes for discovery and discrimination of lipolytic and esterolytic enzymes in mouse liver subproteomes. Proteomes labeled with the fluorescent probes were analyzed by 2-DE while proteomes labeled with the biotinylated probe were subjected to avidin-affinity isolation. Of 37 totally identified proteins, 15 were detected using both approaches while 14 and 8 were solely identified by 2-DE and avidin-affinity isolation, respectively. Moreover, 12 enzymes were classified as potential lipases and/or cholesteryl esterases by their reaction with probes specific for the respective activities directly in their proteomes.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200800191