Molecular Diagnosis of Sentinel Lymph Node Metastases in Cervical Cancer Using Squamous Cell Carcinoma Antigen

• Published in: Clinical cancer research 2008-09, Vol.14 (17), p.5571-5578
• Main Authors: YUAN, Song-Hua, LIANG, Xue-Fang, JIA, Wei-Hua, HUANG, Jian-Ling, MEI WEI, LING DENG, LIANG, Li-Zhi, WANG, Xiao-Yun, ZENG, Yi-Xin
• Format: Article
• Language: English
• Subjects: Adult; Antigens, Neoplasm - analysis; Antigens, Neoplasm - genetics; Antineoplastic agents; Biological and medical sciences; cervical cancer; Female; Female genital diseases; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Gynecology. Andrology. Obstetrics; Hematologic and hematopoietic diseases; Humans; Keratin-19 - genetics; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; lymphatic metastasis; Lymphatic Metastasis - genetics; Medical sciences; Middle Aged; molecular diagnosis; Pharmacology. Drug treatments; Polymerase Chain Reaction - methods; RNA, Messenger - analysis; Sentinel Lymph Node Biopsy; Serpins - analysis; Serpins - genetics; Tumors; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-08-0346

Purpose: To clarify the prognostic value of molecular diagnosis of SLN metastases in cervical cancer using SCCA . Experimental Design: All SLNs and primary tumors, part of non-SLNs, were harvested from 36 patients with cervical cancer. Expression levels of SCCA , cytokeratin 19 ( CK19 ), and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) mRNA in 178 samples (29 primary tumors, 5 histologic positive nodes, 60 histologic negative SLNs, 69 non-SLNs, and 15 normal nodes) were assessed by quantitative reverse transcription-PCR assay. The quantitative value of SCCA or CK19 mRNA was described as each value relative to GAPDH mRNA. The cutoff value was set at the upper limit of the quantitative value of nodes from noncancer patients, and those above this value constituted the molecular metastasis group. Results: The SCCA mRNA expression values were more than 1 × 10 3 in 28 primary tumors and all histologic positive nodes, and its expression levels in SLNs were higher than in non-SLNs. SLNs from patients with adverse prognostic features had higher SCCA mRNA expression levels. Four histologic negative SLNs were diagnosed molecular metastases based on SCCA mRNA. Two cases with histologically uninvolved pelvic nodes recurred. Survival analysis indicates that molecular lymphatic metastasis based on elevated SCCA mRNA level is the best predictor of recurrence. However, CK19 is not a suitable marker due to its low specificity and relative higher baseline expression in normal nodes. Conclusions: SCCA mRNA levels for molecular diagnosis of SLN metastases in cervical cancer more accurately identifies patients at risk for recurrence than the routine histology does.