Use of experimental design and effective mobility calculations to develop a method for the determination of antimicrobials by capillary electrophoresis

A capillary zone electrophoresis (CZE) method for the determination of chloramphenicol (CLP), danofloxacin (DANO), ciprofloxacin (CIPRO), enrofloxacin (ENRO), sulfamethazine (SMZ), sulfaquinoxaline (SQX) and sulfamethoxazole (SMX) is described. For the development, the effective mobilities were esti...

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Bibliographic Details
Published in:Talanta (Oxford) 2008-09, Vol.76 (5), p.1006-1014
Main Authors: Mamani, Mónica Cecília Vargas, Amaya-Farfan, Jaime, Reyes, Felix Guillermo Reyes, Silva, José Alberto Fracassi da, Rath, Susanne
Format: Article
Language:English
Subjects:
Analytical chemistry
Anti-Infective Agents - analysis
Capillary zone electrophoresis
Chemistry
Chloramphenicol
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid
Effective mobilities
Electrophoresis, Capillary - methods
Exact sciences and technology
Experimental design
Fluoroquinolones
Other chromatographic methods
Reproducibility of Results
Sulfonamides
Time Factors
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Summary:A capillary zone electrophoresis (CZE) method for the determination of chloramphenicol (CLP), danofloxacin (DANO), ciprofloxacin (CIPRO), enrofloxacin (ENRO), sulfamethazine (SMZ), sulfaquinoxaline (SQX) and sulfamethoxazole (SMX) is described. For the development, the effective mobilities were estimated and a central composite design was performed. The method was in-house validated for CLP, CIPRO, ENRO and SMX determination in pharmaceuticals. In comparison with the HPLC method recommended by the United States Pharmacopoeia, this CZE method exhibited the same performance, with the advantage that seven different antimicrobials in pharmaceutical formulations could be simultaneously determined.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2008.04.062