Biometrical and genetical characterization of large Babesia Ovis in Iran

One species of Babesia was identified on the blood smear of 20 different naturally infected sheep in the Northwest of Iran. It was polymorphic, including double pyriform with acute or obtuse angle, single pyriform, and ring form. The size of typical paired pyriforms with acute angle was 2.7 x 0.4 μm...

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Bibliographic Details
Published in:Parasitology research (1987) 2008-06, Vol.103 (1), p.217-221
Main Authors: Shayan, Parviz, Hooshmand, Elham, Nabian, Sedighe, Rahbari, Sadegh
Format: Article
Language:English
Subjects:
Animals
Babesia
Babesia - genetics
Babesiosis - parasitology
Babesiosis - veterinary
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Experimental infection
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Immunology
Invertebrates
Iran - epidemiology
Medical Microbiology
Microbiology
Morphology
Original Paper
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
Restriction fragment length polymorphism
rRNA 18S
Sensitivity and Specificity
Sequence analysis
Sheep
Sheep Diseases - epidemiology
Sheep Diseases - parasitology
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Summary:One species of Babesia was identified on the blood smear of 20 different naturally infected sheep in the Northwest of Iran. It was polymorphic, including double pyriform with acute or obtuse angle, single pyriform, and ring form. The size of typical paired pyriforms with acute angle was 2.7 x 0.4 μm (n = 10) and with obtuse angle was 3.5 x 0.6 μm (n = 10). Although the morphological and biometrical parameters resembled the Babesia motasi, the results of seminested polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism using primers specific for small subunit of 18S rRNA confirmed this species as Babesia ovis. Furthermore, the sequence analysis of hypervariable region of small subunit of 18S rRNA revealed the corresponding sequences for B. ovis as well. Experimental infection of healthy lambs with the morphological larger B. ovis showed a milder clinical signs compared to the small one.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-008-0960-1