Preliminary study on genetic variability of Dicrocoelium dendriticum determined by random amplified polymorphic DNA

Genetic variability of adult specimens of Dicrocoelium dendriticum has been studied using random amplified polymorphic DNA (RAPD). The worms were collected from the infected livers of different sheep from several localities in León province (NW Spain). DNA fragments were amplified by means of decame...

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Bibliographic Details
Published in:Parasitology international 1999-03, Vol.48 (1), p.21-26
Main Authors: Sandoval, Hilda, Manga-González, Yolanda, Campo, Raquel, Garcı́a, Pedro, Castro, José Marı́a, de la Vega, Marcelino Pérez
Format: Article
Language:English
Subjects:
Animals
Dicrocoeliasis - parasitology
Dicrocoeliasis - veterinary
Dicrocoelium - genetics
Dicrocoelium dendriticum
DNA, Helminth - analysis
Genes, Helminth
Genetic variability
Genetic Variation - genetics
Random amplified polymorphic DNA (RAPD)
Random Amplified Polymorphic DNA Technique - methods
Sheep
Sheep Diseases - parasitology
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Summary:Genetic variability of adult specimens of Dicrocoelium dendriticum has been studied using random amplified polymorphic DNA (RAPD). The worms were collected from the infected livers of different sheep from several localities in León province (NW Spain). DNA fragments were amplified by means of decamer primer oligonucleotides of arbitrary sequence. Some primers produce complex and highly variable patterns of amplified DNA in D. dendriticum. Intra- and inter-population genetic variability of adult parasites were analyzed, scoring polymorphic and monomorphic reproducible bands by means of the Jaccard similarity, and dendrograms showing genetic relationships between individuals were obtained using the FITCH method. Genetic variability seems to be high in this parasite and genetic similarity within a population (worms infecting a single animal) is similar to the average similarity between worms from different sheep. These results suggest that each sheep is infected by numerous genetically different parasites from one or more populations of infected ants.
ISSN:1383-5769
1873-0329
DOI:10.1016/S1383-5769(98)00035-X