The role of CD11b in phagocytosis and dendritic cell development

Abstract Activation of resting T cells is highly dependent on dendritic cells (DCs), which take up antigens and present antigenic peptides to T cells in the context of the major histocompatibility complex (MHC). In this study, we generated a monoclonal antibody, which we call 1C4 that recognizes int...

Full description

Saved in:
Bibliographic Details
Published in:Immunology letters 2008-09, Vol.120 (1), p.42-48
Main Authors: Chen, Jingtao, Namiki, Sahori, Toma-Hirano, Makiko, Miyatake, Shoichiro, Ishida, Koji, Shibata, Yasue, Arai, Naoko, Arai, Ken-ichi, Kamogawa-Schifter, Yumiko
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Monoclonal - immunology
Antigen presentation
Apoptosis
Apoptosis - immunology
CD11b Antigen - immunology
Cell Count
Cell Differentiation - immunology
Dendritic Cells - cytology
Female
Integrin
Macrophage-1 Antigen - immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Phagocytosis
Phagocytosis - immunology
Rats
Rats, Wistar
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Activation of resting T cells is highly dependent on dendritic cells (DCs), which take up antigens and present antigenic peptides to T cells in the context of the major histocompatibility complex (MHC). In this study, we generated a monoclonal antibody, which we call 1C4 that recognizes integrin αM β2 (CD11b/CD18) on the surface of conventional DCs (cDCs) and is internalized after binding. Addition of 1C4 inhibited the ability of immature DCs to phagocytose apoptotic cells. 1C4 treatment also partially inhibited the generation of cDCs from bone marrow in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF). Our findings suggest that not only CD11b is involved in the phagocytosis of apoptotic cells, but also that mAb such as 1C4 may be a useful tool for the delivery of specific proteins into the cytoplasm of immature DCs.
ISSN:0165-2478
1879-0542
DOI:10.1016/j.imlet.2008.06.010