Tuning of Thermo-responsive Self-Assembly Monolayers on Gold for Cell-Type-Specific Control of Adhesion

Self-assembled monolayers (SAMs) on gold containing a thermo-responsive poly(N-isopropylacrylamide)-poly(ethylene glycol)-thiol copolymer were formed. These layers show considerable potential for inducing enzyme-free and gentle detachment of cultivated cells. In an effort to optimize detachment of c...

Full description

Saved in:
Bibliographic Details
Published in:Langmuir 2008-09, Vol.24 (18), p.10259-10264
Main Authors: Ernst, Oliver, Lieske, Antje, Holländer, Andreas, Lankenau, Andreas, Duschl, Claus
Format: Article
Language:English
Subjects:
Adhesiveness
Animals
Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials
Cell Adhesion
Cell Line, Tumor
Chemistry
Colloidal state and disperse state
Exact sciences and technology
General and physical chemistry
Gold - chemistry
Humans
Mice
Microscopy, Phase-Contrast
Osteosarcoma - metabolism
Polyethylene Glycols - chemistry
Polymers - chemistry
Spectrometry, X-Ray Emission - methods
Sulfhydryl Compounds
Surface physical chemistry
Surface Properties
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Self-assembled monolayers (SAMs) on gold containing a thermo-responsive poly(N-isopropylacrylamide)-poly(ethylene glycol)-thiol copolymer were formed. These layers show considerable potential for inducing enzyme-free and gentle detachment of cultivated cells. In an effort to optimize detachment of cells, including strongly adhering ones, two approaches are presented. First, two thermo-responsive copolymers with different poly(ethylene glycol) (PEG) contents of 15 wt % (“P15”) and 19 wt % (“P19”) were grafted to Au surfaces. Second, mixed monolayers were formed containing P19 and various concentrations of thiol bearing PEG. X-ray photoelectron spectroscopy (XPS) on pure and mixed P19 containing layers confirmed the expected layer compositions. Contact angle measurements showed good functionality of all surfaces prepared. Upon a temperature decrease below the lower critical solution temperature (LCST), the duration until cultivated fibroblasts detached from pure P19 surfaces was half of the one determined on P15. Strongly adherent human osteosarcoma cells could not be detached from pure P19 layers. Through co-adsorption of P19 and thiol-bearing PEG of a molar composition of 1:6, layers were formed that allowed good spreading of osteosarcoma cells above LCST and their efficient detachment below LCST.
ISSN:0743-7463
1520-5827
DOI:10.1021/la801026y