Mixing of M segment DNA vaccines to Hantaan virus and Puumala virus reduces their immunogenicity in hamsters

Abstract To determine if DNA vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, Hantaan virus and Puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. Both vaccines elicited neut...

Full description

Saved in:
Bibliographic Details
Published in:Vaccine 2008-09, Vol.26 (40), p.5177-5181
Main Authors: Spik, Kristin W, Badger, Catherine, Mathiessen, Iacob, Tjelle, Torunn, Hooper, Jay W, Schmaljohn, Connie
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies, Viral - blood
Applied microbiology
Biolistics
Biological and medical sciences
Cercopithecus aethiops
COS Cells
Cricetinae
Deoxyribonucleic acid
DNA
DNA vaccines
Drug Therapy, Combination
Ebola virus
Electroporation
Encephalitis
Fever
Fundamental and applied biological sciences. Psychology
Genomes
Hantaan virus
Hantaan virus - genetics
Hantaan virus - immunology
Hantaviruses
Hemorrhagic fever with renal syndrome
Hemorrhagic Fever with Renal Syndrome - immunology
Hemorrhagic Fever with Renal Syndrome - prevention & control
Hemorrhagic Fever with Renal Syndrome - virology
Immunogenicity
Microbiology
Miscellaneous
Neutralization Tests
Plasmids
Puumala virus
Puumala virus - genetics
Puumala virus - immunology
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, DNA - administration & dosage
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Vero Cells
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract To determine if DNA vaccines for two hantaviruses causing hemorrhagic fever with renal syndrome, Hantaan virus and Puumala virus, are immunogenic when given in combination, we delivered them to hamsters separately or as mixtures by gene gun or by electroporation. Both vaccines elicited neutralizing antibodies when given alone but when they were delivered as a mixture, antibodies to only one of the two hantaviruses could be detected. In contrast, if the DNAs were given as separate vaccinations to a single animal, responses to both were observed. These studies suggest that the two DNA vaccines will need to be given as separate administrations.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2008.03.097