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Sperm subpopulations in boar (Sus scrofa) and gazelle (Gazella dama mhorr) semen as revealed by pattern analysis of computer-assisted motility assessments
The aim of this study was to test the suitability of âpattern analysisâ for the exploration of data provided by computer-assisted semen analysis methods. Data sets derived from the examination of boar sperm responses to bicarbonate and caffeine (measurements on 3208 spermatozoa) and from studies...
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Published in: | Biology of reproduction 1999, Vol.60 (1), p.32-41 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The aim of this study was to test the suitability of âpattern analysisâ for the exploration of data provided by computer-assisted
semen analysis methods. Data sets derived from the examination of boar sperm responses to bicarbonate and caffeine (measurements
on 3208 spermatozoa) and from studies of semen cryopreservation in Mohor gazelles (7278 spermatozoa) were reanalyzed. A nonhierarchical
classification method was used to generate initial subgroups of spermatozoa (9 for boar, 13 for gazelle). The subgroup centroids
were fused, yielding three boar sperm subpopulations and four gazelle sperm subpopulations distinguished by sperm behaviors.
Bicarbonate and caffeine both induced major transitions ( p < 0.0001) of boar sperm behavior, detected as shifts in group membership (from group 2, i.e., active but nonlinear movement,
into group 1, i.e., linear, rapid movement). Some spermatozoa (approximately 3%) were refractory to both caffeine and bicarbonate.
The gazelle sperm subpopulation structure was affected by the inclusion of equex (sodium triethanolamine lauryl sulfate) in
the cryoprotective diluents. Equex suppressed the appearance of spermatozoa with erratic behavior ( p < 0.0001; high curvilinear velocity, low linearity, low straight-line velocity) after cryopreservation. The proportion of
these erratic spermatozoa was positively correlated with animal age ( r = 0.68, p = 0.029). Pattern analysis revealed novel aspects of the data not seen in the original investigations and usefully supplemented
the more standard data analysis approaches. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod60.1.32 |