Immune-expression of HSP27 and IL-10 in recurrent aphthous ulceration

• Published in: Journal of oral pathology & medicine 2008-09, Vol.37 (8), p.462-467
• Main Authors: Miyamoto Jr, Nelson T., Borra, Ricardo Carneiro, Abreu, Marilda, Weckx, Luc Louis Maurice, Franco, Marcello
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal; Biological and medical sciences; Colitis, Ulcerative - immunology; Colitis, Ulcerative - pathology; Coloring Agents; Crohn Disease - immunology; Crohn Disease - pathology; Cytoplasm - immunology; Cytoplasm - ultrastructure; Dentistry; Epithelial Cells - immunology; Epithelial Cells - pathology; Extracellular Space - immunology; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Fibrosis; heat shock protein 27; HSP27 Heat-Shock Proteins - analysis; Humans; Hyperplasia; Immunoenzyme Techniques; Immunohistochemistry; interleukin 10; Interleukin-10 - analysis; Medical sciences; Mouth Mucosa - immunology; Mouth Mucosa - pathology; Non tumoral diseases; Otorhinolaryngology. Stomatology; Recurrence; recurrent aphthous ulceration; Stomatitis, Aphthous - immunology; Stomatitis, Aphthous - pathology
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/j.1600-0714.2008.00665.x

Background:  Recently, abnormal cellular immune response has been considered responsible for the oral lesion in the recurrent aphthous ulceration (RAU). For reasons not yet defined, antigens of the oral microbiota would trigger abnormal Th1 immune response against epithelial cells. On the other hand, studies have demonstrated that heat shock proteins (HSP) can block the production of proinflammatory cytokine through inhibition of NF‐κB and mitogen‐activated protein kinase pathways or activate anti‐inflammatory cytokines and therefore control the magnitude of the immune response. HSP27 has been considered a powerful inductor of IL‐10, a major inhibitor of Th1 response. Methods:  Using immunohistochemistry, we studied the expression and location of HSP27 and IL‐10 in ulcerated lesions clinically diagnosed as RAU (n = 27) and to compare it with that of oral clinically normal mucosa (CT; n = 6) and of other inflammatory chronic diseases such as oral fibrous inflammatory hyperplasia (FIH; n = 18), Crohn’s disease (CD; n = 10) and ulcerative colitis (UC; n = 9). Results:  A lower proportion of HSP27‐positive epithelial cells in RAU and CD were observed when compared with CT and FIH (P