Inhibitory Effects of Tunisian Marine Algal Extracts on Digestive Lipases

The lipase inhibitory activity of ethanol extracts obtained from some marine algae collected on the Tunisian coast was evaluated. Caulerpa prolifera extract markedly reduced both dog gastric (DGL) and human pancreatic lipase (HPL) activities. Generally, the inhibition reached 100% after 40 to 60 min...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2008-10, Vol.151 (1), p.71-79
Main Authors: Ben Rebah, Faouzi, Smaoui, Sana, Frikha, Fakher, Gargouri, Youssef, Miled, Nabil
Format: Article
Language:English
Subjects:
Algae
Animals
bile salts
Biochemistry
Biological and medical sciences
Biotechnology
Caulerpa
Caulerpa prolifera
Chemistry
Chemistry and Materials Science
Digestive system
Dogs
enzyme inactivation
enzyme inhibition
Ethanol
Eukaryota - chemistry
Fractionation
Fundamental and applied biological sciences. Psychology
Gastric Juice - chemistry
Gastric Juice - enzymology
humans
Inhibitor drugs
Lipase - antagonists & inhibitors
Lipids
obesity
Oceans and Seas
pancreas
Pancreatic Juice - chemistry
Pancreatic Juice - enzymology
plant extracts
synergism
Thin layer chromatography
triacylglycerol lipase
Tunisia
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Summary:The lipase inhibitory activity of ethanol extracts obtained from some marine algae collected on the Tunisian coast was evaluated. Caulerpa prolifera extract markedly reduced both dog gastric (DGL) and human pancreatic lipase (HPL) activities. Generally, the inhibition reached 100% after 40 to 60 min of incubation depending on lipase types and on substrates used. Moreover, the inhibitory effect of C. prolifera extract on lipases appeared to be accelerated by adding bile salts, which likely modified the interface and allowed the inhibitory compound to inactivate the lipase. The separation of C. prolifera extract by thin-layer chromatography (TLC) resulted in eight fractions showing efficient inhibition rate against DGL, compared to the crude extract. In the case of HPL, TLC fractionation reduced the inhibitory rates, suggesting that the effect of algal extract on lipases may be caused by a synergetic action of several compounds within the extract. High-performance liquid chromatograph separation resulted in isolation of a major compound displaying high inhibition capacity of HPL activity. Caulerpa prolifera extract may therefore be useful in developing antiobesity drugs.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-008-8167-6