Distribution of plasmid-borne stress protein genes in Streptococcus thermophilus and other lactic acid bacteria

The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an...

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Bibliographic Details
Published in:Current microbiology 1999, Vol.38 (1), p.43-47
Main Authors: Somkuti, G.A, Steinberg, D.H
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteria
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Blotting, Southern
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genes
Genes, Bacterial
Heat tolerance
Heat-Shock Proteins - genetics
Lactobacillus
Lactobacillus - genetics
Lactococcus
Lactococcus - genetics
Leuconostoc
Leuconostoc - genetics
Microbiology
Nucleic Acid Hybridization
Plasmids
Streptococcus
Streptococcus - chemistry
Streptococcus - genetics
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Summary:The presence of heat stress protein genes (hsp) was tested by Southern hybridization analysis in total DNA extracts from species of the genus Streptococcus (47 strains), Lactobacillus (34 strains), Lactococcus (24 strains), and Leuconostoc (5 strains). The biotinylated hsp16.4 probe prepared from an ORF2 fragment of pER341 (2.8 kb) tested positively with restricted DNA extracts of seven Streptococcus thermophilus strains and a single strain of Lactococcus lactis subsp. cremoris. In all positive S. thermophilus strains, the hsp was located on plasmids ranging from cat 2.8 kb to 11 kb in size, while hsp was present in a 7.5-kb plasmid in Lactococcus lactis subsp. cremoris. Southern blots with a rep probe showed that all hsp16.4+ plasmids in S. thermophilus strains also shared homology with the replication function (rep) of pER341, suggesting the common origin of these plasmids.
ISSN:0343-8651
1432-0991
DOI:10.1007/PL00006770