Effect of postischaemic hypothermia on the mitochondrial damage induced by ischaemia and reperfusion in the gerbil

In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37°C or 30°C. After normothermic (37°C) ischaemia/reperfusion, significant decreases...

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Bibliographic Details
Published in:Brain research 1999-01, Vol.817 (1-2), p.241-245
Main Authors: Canevari, Laura, Console, Antonella, Tendi, Elisabetta A, Clark, John B, Bates, Timothy E
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Biological and medical sciences
Brain Ischemia - pathology
Brain Ischemia - therapy
Cerebral ischemia
Cytochrome c oxidase
Gerbillinae
Hypothermia
Hypothermia, Induced
Medical sciences
Mitochondria
Mitochondria - pathology
Neurology
Oxygen Consumption - physiology
Reperfusion
Reperfusion Injury - pathology
Respiratory chain
Synaptosomes - pathology
Vascular diseases and vascular malformations of the nervous system
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Summary:In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37°C or 30°C. After normothermic (37°C) ischaemia/reperfusion, significant decreases in mitochondrial state 3 (+ADP) oxygen consumption (−42.2%), complex II–III activity in synaptosomes (−31.7%) and complex IV were measured, in both free mitochondria and synaptosomes (−30.3% and −27.8% respectively). However, following hypothermic (30°C) reperfusion, both respiration rates and all enzyme activities remained at levels not significantly different from those in the sham operated controls.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(98)01278-5