In Vivo Characterization of Chimeric Phytochromes in Yeast

Phytochromes are plant photoreceptors that play a major role in photomorphogenesis. Two members of the phytochrome family have been characterized in some detail. Phytochrome A, which controls very low fluence and high irradiance responses, is rapidly degraded in the light, forms sequestered areas of...

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Bibliographic Details
Published in:The Journal of biological chemistry 1999-01, Vol.274 (1), p.354-359
Main Authors: Eichenberg, Klaus, Kunkel, Tim, Kretsch, Thomas, Speth, Volker, Schäfer, Eberhard
Format: Article
Language:English
Subjects:
APOPROTEINAS
APOPROTEINE
APOPROTEINS
CHEMICAL REACTIONS
Cloning, Molecular
DARK REVERSION
DARKNESS
ESPECTROMETRIA
FITOCROMA
GENE TRANSFER
GENETIC TRANSFORMATION
IMMUNOCYTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
INMUNOLOGIA
Kinetics
NICOTIANA TABACUM
OBSCURIDAD
OBSCURITE
ORYZA SATIVA
PHYCOCYANOBILIN
PHYTOCHROME
Phytochrome - chemistry
Phytochrome - genetics
PIGMENT
PIGMENTOS
PIGMENTS
PROTEINAS RECOMBINANTES
PROTEINE RECOMBINANTE
REACCIONES QUIMICAS
REACTION CHIMIQUE
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - genetics
RECOMBINANT PROTEINS
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
SPECTRAL DATA
SPECTROMETRIE
SPECTROMETRY
Spectrum Analysis
TRANSFERENCIA DE GENES
TRANSFERT DE GENE
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
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Summary:Phytochromes are plant photoreceptors that play a major role in photomorphogenesis. Two members of the phytochrome family have been characterized in some detail. Phytochrome A, which controls very low fluence and high irradiance responses, is rapidly degraded in the light, forms sequestered areas of phytochrome (SAPs), and does not exhibit dark reversion in monocotyledonous seedlings. Phytochrome B mediates red/far-red reversible responses, is stable in the light, and does not form SAPs. We report on the behavior in yeast of the phytochrome apoproteins of rice PHYA, tobacco PHYB, and chimeric PHYAB and PHYBA and on the behavior of the respective holoprotein adducts after assembly with phycocyanobilin chromophore (PHY*). SAP-like formation in yeast was not observed for PHYB, but was detectable for PHYA, PHYAB, and PHYBA. Rice PHYA* did not undergo dark reversion in yeast. Surprisingly, all other tested phytochrome constructs did exhibit dark reversion, including chimeric phytochromes with a short N-terminal part of tobacco PHYB or parsley PHYA fused to rice PHYA. Furthermore, the proportion of phytochrome undergoing dark reversion and the rate of reversion were increased for both the N terminus-swapped constructs and PHYBA*. These results are discussed with respect to structure/function analysis of phytochromes A and B.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.274.1.354