Unilateral joint inflammation induces bilateral and time-dependent changes in neuropeptide FF binding in the superficial dorsal horn of the rat spinal cord: implication of supraspinal descending systems

• Published in: Brain research 1999-01, Vol.816 (2), p.598-608
• Main Authors: Lombard, Marie-Christine, Weil-Fugazza, Jeanne, Ries, Colette, Allard, Michèle
• Format: Article
• Language: English
• Subjects: Animals; Arthritis, Experimental - metabolism; Biological and medical sciences; Chronic pain; Descending inhibitory control; Functional Laterality - physiology; Fundamental and applied biological sciences. Psychology; Male; Monoarthritis; Neural Inhibition - physiology; Neuropeptide FF; Neuropeptides - metabolism; Nociception; Oligopeptides - metabolism; Radioligand Assay; Rat spinal cord; Rats; Rats, Sprague-Dawley; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors; Spinal Cord - metabolism; Time Factors; Vertebrates: nervous system and sense organs
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(98)01242-6

Using quantitative autoradiography, the effects of acute and chronic inflammation on specific 125 I -1DMethyl-FLFQPQRFamide binding were investigated in the rat spinal cord dorsal horn superficial layers, at 6 and 24 h and 2, 4, 6 and 12 weeks after induction of monoarthritis produced by injection of killed Mycobacterium butyricum suspended in Freund adjuvant in one tibio-tarsal joint. Six hours after monoarthritis induction, no modification in specific 125 I -1DMethyl-FLFQPQRFamide binding was observed, whereas a significant bilateral increase occurred after 24 h and 2 weeks in L4/L5 dorsal horns, with a return to control values at 4, 6 and 12 weeks. Specific 125 I -1DMethyl-FLFQPQRFamide binding was also investigated 24 h after monoarthritis induction in rats submitted 4 days before the induction to spinal cord lesions at the thoracic level (T9–T10). Hemisection of the spinal cord contralateral to the affected ankle prevented the transient bilateral increase in specific 125 I -1DMethyl-FLFQPQRFamide binding, whereas total spinal cord section induced a significant bilateral decrease. All of these modifications were restricted to the spinal segments receiving afferent input from the arthritic ankle (L4/L5); no modifications were found at the levels L1 or C6–C8. These data suggest that FLFQPQRFamide is involved in spinal nociceptive processing during sustained peripheral nociceptor activation. The effects of spinal cord lesions in monoarthritic rats indicate that the modifications seen in the FLFQPQRFamide system activity, during sustained peripheral inflammation, depend on afferent fiber activation as well as on supraspinal controls.